Suppression of murine experimental autoimmune encephalomyelitis development by 1,25-dihydroxyvitamin D3 with autophagy modulation.

Suppression of murine experimental autoimmune encephalomyelitis development by 1,25-dihydroxyvitamin D3 with autophagy modulation.

Journal of neuroimmunology (2015-03-17)

Chao Zhen, Xuedan Feng, Zhe Li, Yabo Wang, Bin Li, Lin Li, Moyuan Quan, Gaoning Wang, Li Guo

ABSTRACT

Multiple sclerosis (MS) has been associated with a history of sub-optimal exposure to ultraviolet light, implicating vitamin D3 as a possible protective agent. We evaluated whether 1,25(OH)2D3 attenuates the progression of experimental autoimmune encephalomyelitis (EAE), and explored its potential mechanisms. EAE was induced in C57BL/6 mice via immunization with MOG35-55, and some mice received 1,25(OH)2D3. 1,25(OH)2D3 inhibited EAE progression. Additionally, 1,25(OH)2D3 reduced inflammation, demyelination, and neuron loss in the spinal cord. The protective effect of 1,25(OH)2D3 was associated with significantly elevated expression of Beclin1, increased Bcl-2/Bax ratio, and decreased LC3-II accumulation. Thus, 1,25(OH)2D3 may represent a promising new MS treatment.

MATERIALS

Product Number

Brand

Product Description

436143

Sigma-Aldrich

Sodium dodecyl sulfate, ACS reagent, ≥99.0%

71729

Sigma-Aldrich

Sodium dodecyl sulfate, ≥98.0% (GC)

71726

Supelco

Sodium dodecyl sulfate, suitable for ion pair chromatography, LiChropur^™, ≥99.0%

62862

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Sodium dodecyl sulfate, ≥90% ((Assay))

L6026

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Sodium dodecyl sulfate, BioXtra, ≥99.0% (GC)

L4509

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Sodium dodecyl sulfate, ReagentPlus^®, ≥98.5% (GC)

L5750

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Sodium dodecyl sulfate, 92.5-100.5% based on total alkyl sulfate content basis

D8284

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Bicinchoninic acid disodium salt hydrate, ≥98% (HPLC)

L3771

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Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

71717

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Sodium dodecyl sulfate, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate

28-3260

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Sodium dodecyl sulfate, SAJ special grade, ≥97.0%

71725

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Sodium dodecyl sulfate, BioUltra, for molecular biology, ≥99.0% (GC)

75746

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Sodium dodecyl sulfate, ≥99.0% (GC), dust-free pellets

28-3285

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Sodium dodecyl sulfate, ≥99.0%

C0225000

Calcitriol, European Pharmacopoeia (EP) Reference Standard

RDD040

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Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC), free-flowing, Redi-Dri^™

74255

Supelco

Sodium dodecyl sulfate, dust-free pellets, suitable for electrophoresis, for molecular biology, ≥99.0% (GC)

28-0020

Sigma-Aldrich

Sucrose, SAJ first grade

739898

Sigma-Aldrich

1α,25-Dihydroxyvitamin D₂ solution, 50 μg/mL in ethanol, 95% (CP)

PHR1001

Supelco

Sucrose, Pharmaceutical Secondary Standard; Certified Reference Material

739677

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1α,25-Dihydroxyvitamin D₂ solution, 100 μg/mL in ethanol, 95% (CP)

1623637

USP

Sucrose, United States Pharmacopeia (USP) Reference Standard

05030

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Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 20% in H₂O

16104

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Sucrose, puriss., meets analytical specification of Ph. Eur., BP, NF

84097

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Sucrose, BioUltra, for molecular biology, ≥99.5% (HPLC)

84100

Millipore

Sucrose, suitable for microbiology, ACS reagent, ≥99.0%

71736

Sigma-Aldrich

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D1530

Sigma-Aldrich

1α,25-Dihydroxyvitamin D₃, ≥99% (HPLC)

S7903

Sigma-Aldrich

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S9378

Sigma-Aldrich

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