Skip to Content
MilliporeSigma
  • The role of the Syk/Shp-1 kinase-phosphatase equilibrium in B cell development and signaling.

The role of the Syk/Shp-1 kinase-phosphatase equilibrium in B cell development and signaling.

Journal of immunology (Baltimore, Md. : 1950) (2014-06-06)
Ameera Alsadeq, Elias Hobeika, David Medgyesi, Kathrin Kläsener, Michael Reth
ABSTRACT

Signal transduction from the BCR is regulated by the equilibrium between kinases (e.g., spleen tyrosine kinase [Syk]) and phosphatases (e.g., Shp-1). Previous studies showed that Syk-deficient B cells have a developmental block at the pro/pre-B cell stage, whereas a B cell-specific Shp-1 deficiency promoted B-1a cell development and led to autoimmunity. We generated B cell-specific Shp-1 and Syk double-knockout (DKO) mice and compared them to the single-knockout mice deficient for either Syk or Shp-1. Unlike Syk-deficient mice, the DKO mice can generate mature B cells, albeit at >20-fold reduced B cell numbers. The DKO B-2 cells are all Syk-negative, whereas the peritoneal B1 cells of the DKO mice still express Syk, indicating that they require this kinase for their proper development. The DKO B-2 cells cannot be stimulated via the BCR, whereas they are efficiently activated via TLR or CD40. We also found that in DKO pre-B cells, the kinase Zap70 is associated with the pre-BCR, suggesting that Zap70 is important to promote B cell maturation in the absence of Syk and SHP-1. Together, our data show that a properly balanced kinase/phosphatase equilibrium is crucial for normal B cell development and function.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium chloride solution, BioUltra, for molecular biology, ~5 M in H2O
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride, BioUltra, for molecular biology, ≥99.5% (AT)
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride, tested according to Ph. Eur.
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride solution, 0.85%
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride, random crystals, optical grade, 99.9% trace metals basis
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride, 99.999% trace metals basis
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride-35Cl, 99 atom % 35Cl
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride, AnhydroBeads, −10 mesh, 99.999% trace metals basis
Pricing and availability is not currently available.
Supelco
Sodium chloride, reference material for titrimetry, certified by BAM, >99.5%
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride, tablet
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride, BioXtra, ≥99.5% (AT)
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride solution, 5 M
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride, JIS special grade, ≥99.5%
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride, SAJ first grade, ≥99.0%
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride solution, 1 M
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride solution, 0.1 M
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium chloride, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture
Pricing and availability is not currently available.
Supelco
Sodium chloride, Pharmaceutical Secondary Standard; Certified Reference Material
Pricing and availability is not currently available.
SAFC
Sodium chloride solution, 5 M
Pricing and availability is not currently available.