Skip to Content
MilliporeSigma
  • Genotypic variation and antifungal susceptibly of Candida zeylanoides clinical isolates.

Genotypic variation and antifungal susceptibly of Candida zeylanoides clinical isolates.

Journal de mycologie medicale (2014-04-22)
H Shokri
ABSTRACT

To investigate the DNA fingerprinting of Candida zeylanoides (C. zeylanoides) strains and the correlation between genotyping and antifungal susceptibility of C. zeylanoides. Paper discs containing nystatin, ketoconazole, fluconazole, Zataria multiflora (Z. multiflora) and Pulicaria gnaphalodes (P. gnaphalodes) essential oils were used in the disc diffusion method to evaluate the in vitro activity of the antifungal agents by measuring the mean diameter of inhibition around the discs. Yeast isolates were characterized by randomly amplified polymorphic DNA (RAPD) analysis using two different primers. The mean inhibition zones were calculated 36.1 ± 2.2mm for ketoconazole, 25.3 ± 2.0mm for nystatin and 14.9 ± 1.4mm for fluconazole. Z. multiflora essential oil revealed a 58.6 ± 2.6mm mean zone of inhibition while P. gnaphalodes showed a 36.7 ± 1.8mm zone of inhibition against all isolates tested. A total of 14 strains of C. zeylanoides were divided into three types, 1 was genotype A strain, 2 genotype B strains and 11 genotype C strains. Strain of genotype A was significantly more susceptible to ketoconazole and nystatin than fluconazole (P<0.05). Strains of genotype B were more susceptible to ketoconazole than other antifungal agents (P>0.05). Strains of genotype C were significantly more susceptible to ketoconazole than fluconazole and nystatin (P<0.05). There was a significant correlation between C. zeylanoides genotypes and antifungal susceptibility (P < 0.05). We concluded that all C. zeylanoides genotypes were susceptible to nystatin and Z. multiflora essential oil. The correlation between antifungal susceptibility and C. zeylanoides genotype may be of potential therapeutic significance and larger studies are needed to prove this finding.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Potassium acetate solution, BioUltra, for molecular biology, 5 M in H2O
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Isopropyl alcohol, ≥99.7%, FCC, FG
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Potassium acetate, BioXtra, ≥99.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Sucrose, ACS reagent
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Potassium acetate, meets USP testing specifications
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Sucrose, ≥99.5% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Sucrose, for molecular biology, ≥99.5% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Sucrose, ≥99.5% (GC), BioReagent, suitable for cell culture, suitable for insect cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Sucrose, Grade I, ≥99% (GC), suitable for plant cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Sucrose, ≥99.5% (GC), BioXtra
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Supelco
Sucrose, analytical standard, for enzymatic assay kit SCA20
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Sucrose, ≥99.5% (GC), Grade II, suitable for plant cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Sucrose, ≥99.5% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Sucrose, meets USP testing specifications
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Potassium acetate, for molecular biology, ≥99.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Millipore
Sucrose, suitable for microbiology, ACS reagent, ≥99.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Sucrose, BioUltra, for molecular biology, ≥99.5% (HPLC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Potassium acetate, 98%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Potassium acetate, BioUltra, for molecular biology, ≥99.0% (NT)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Sucrose, puriss., meets analytical specification of Ph. Eur., BP, NF
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Supelco
2-Propanol, analytical standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
2-Propanol, puriss., meets analytical specification of Ph. Eur., BP, USP, ≥99.5% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Potassium acetate, ACS reagent, ≥99.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
2-Propanol, puriss. p.a., ACS reagent, ≥99.8% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Supelco
Sucrose, Pharmaceutical Secondary Standard; Certified Reference Material
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
2-Propanol, ACS reagent, ≥99.5%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Isopropyl alcohol, meets USP testing specifications
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Potassium acetate, puriss., meets analytical specification of Ph. Eur., BP, E261, 99-101%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
2-Propanol, Laboratory Reagent, ≥99.5%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00
Sigma-Aldrich
Potassium acetate, ReagentPlus®, ≥99.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
Please contact Customer Service for Availability
CA$108.00
1 L
Please contact Customer Service for Availability
CA$586.00