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  • Mild alkali-pretreatment effectively extracts guaiacyl-rich lignin for high lignocellulose digestibility coupled with largely diminishing yeast fermentation inhibitors in Miscanthus.

Mild alkali-pretreatment effectively extracts guaiacyl-rich lignin for high lignocellulose digestibility coupled with largely diminishing yeast fermentation inhibitors in Miscanthus.

Bioresource technology (2014-08-01)
Ming Li, Shengli Si, Bo Hao, Yi Zha, Can Wan, Shufen Hong, Yongbo Kang, Jun Jia, Jing Zhang, Meng Li, Chunqiao Zhao, Yuanyuan Tu, Shiguang Zhou, Liangcai Peng
ABSTRACT

In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions. Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold. In contrast, Mfl30 samples with elevated S-levels showed reduced hexose yields compared to the paired sample of 0.89-0.98 folds at p<0.01. Notably, only the G-rich biomass samples exhibited complete enzymatic hydrolysis under 4% NaOH pretreatment. Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation. Therefore, this study proposes an optimal approach for minor genetic lignin modification towards cost-effective biomass process in Miscanthus.

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Ethanol, JIS first grade, 94.8-95.8%
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Ethanol, JIS special grade, 94.8-95.8%
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Dehydrated Alcohol, Pharmaceutical Secondary Standard; Certified Reference Material
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Vanillin melting point standard, Pharmaceutical Secondary Standard; Certified Reference Material
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Ethanol solution, certified reference material, 2000 μg/mL in methanol
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Mettler-Toledo Calibration substance ME 51143093, Vanillin, traceable to primary standards (LGC)
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Dehydrated Alcohol, United States Pharmacopeia (USP) Reference Standard
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Rhein
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Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)
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Vanillin, ReagentPlus®, 99%
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4-Hydroxybenzaldehyde, 98%
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4-Hydroxybenzaldehyde, ≥95.0% (HPLC)
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Vanillin, ≥97%, FCC, FG
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Rhein, technical grade
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Vanillin, natural, ≥97%, FCC, FG
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4-Hydroxybenzaldehyde, ≥97%, FG
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Vanillin, tested according to Ph. Eur.
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4-Hydroxybenzaldehyde, analytical standard
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Vanillin, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
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Vanillin, European Pharmacopoeia (EP) Reference Standard
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Vanillin, Pharmaceutical Secondary Standard; Certified Reference Material
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Vanillin Melting Point Standard, United States Pharmacopeia (USP) Reference Standard
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Benzene, anhydrous, 99.8%
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Benzene, analytical standard
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Ethyl alcohol, Pure, 190 proof, meets USP testing specifications
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Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%
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Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%
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Syringaldehyde, 98%
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Ethanol standards 10% (v/v), 10 % (v/v) in H2O, analytical standard
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Syringaldehyde, ≥98%, FG
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