Skip to Content
MilliporeSigma
  • Binary release of ascorbic acid and lecithin from core-shell nanofibers on blood-contacting surface for reducing long-term hemolysis of erythrocyte.

Binary release of ascorbic acid and lecithin from core-shell nanofibers on blood-contacting surface for reducing long-term hemolysis of erythrocyte.

Colloids and surfaces. B, Biointerfaces (2014-12-02)
Qiang Shi, Qunfu Fan, Wei Ye, Jianwen Hou, Shing-Chung Wong, Xiaodong Xu, Jinghua Yin
ABSTRACT

There is an urgent need to develop blood-contacting biomaterials with long-term anti-hemolytic capability. To obtain such biomaterials, we coaxially electrospin [ascorbic acid (AA) and lecithin]/poly (ethylene oxide) (PEO) core-shell nanofibers onto the surface of styrene-b-(ethylene-co-butylene)-b-styrene elastomer (SEBS) that has been grafted with poly (ethylene glycol) (PEG) chains. Our strategy is based on that the grafted layers of PEG render the surface hydrophilic to reduce the mechanical injure to red blood cells (RBCs) while the AA and lecithin released from nanofibers on blood-contacting surface can actively interact with RBCs to decrease the oxidative damage to RBCs. We demonstrate that (AA and lecithin)/PEO core-shell structured nanofibers have been fabricated on the PEG grafted surface. The binary release of AA and lecithin in the distilled water is in a controlled manner and lasts for almost 5 days; during RBCs preservation, AA acts as an antioxidant and lecithin as a lipid supplier to the membrane of erythrocytes, resulting in low mechanical fragility and hemolysis of RBCs, as well as high deformability of stored RBCs. Our work thus makes a new approach to fabricate blood-contacting biomaterials with the capability of long-term anti-hemolysis.

MATERIALS
Product Number
Brand
Product Description

Supelco
L-Ascorbic acid, analytical standard
Pricing and availability is not currently available.
Supelco
Ethylene glycol, Pharmaceutical Secondary Standard; Certified Reference Material
Pricing and availability is not currently available.
Sigma-Aldrich
Citrate Concentrated Solution, BioUltra, for molecular biology, 1 M in H2O
Pricing and availability is not currently available.
Sigma-Aldrich
L-Ascorbic acid, puriss. p.a., ≥99.0% (RT)
Pricing and availability is not currently available.
Sigma-Aldrich
L-Ascorbic acid, suitable for cell culture, suitable for plant cell culture, ≥98%
Pricing and availability is not currently available.
Sigma-Aldrich
L-Ascorbic acid, FCC, FG
Pricing and availability is not currently available.
Sigma-Aldrich
Ethylene glycol, JIS special grade, ≥99.5%
Pricing and availability is not currently available.
Sigma-Aldrich
Ethylene glycol, SAJ first grade, ≥99.0%
Pricing and availability is not currently available.
Supelco
Ethylene glycol solution, NMR reference standard, 80% in DMSO-d6 (99.9 atom % D), NMR tube size 5 mm × 8 in.
Pricing and availability is not currently available.
USP
Ethylene glycol, United States Pharmacopeia (USP) Reference Standard
Pricing and availability is not currently available.
USP
Dehydrated Alcohol, United States Pharmacopeia (USP) Reference Standard
Pricing and availability is not currently available.
Sigma-Aldrich
L-Ascorbic acid, powder, suitable for cell culture, γ-irradiated
Pricing and availability is not currently available.
Sigma-Aldrich
L-Ascorbic acid, meets USP testing specifications
Pricing and availability is not currently available.
Sigma-Aldrich
L-Ascorbic acid, reagent grade, crystalline
Pricing and availability is not currently available.
Sigma-Aldrich
L-Ascorbic acid, reagent grade
Pricing and availability is not currently available.
Sigma-Aldrich
Citrate Concentrated Solution, BioReagent, suitable for coagulation assays, 4 % (w/v)
Pricing and availability is not currently available.
Sigma-Aldrich
L-Ascorbic acid, BioXtra, ≥99.0%, crystalline
Pricing and availability is not currently available.
Sigma-Aldrich
L-Ascorbic acid, ACS reagent, ≥99%
Pricing and availability is not currently available.
Sigma-Aldrich
L-Ascorbic acid, puriss. p.a., ACS reagent, reag. ISO, Ph. Eur., 99.7-100.5% (oxidimetric)
Pricing and availability is not currently available.
Sigma-Aldrich
L-Ascorbic acid, 99%
Pricing and availability is not currently available.
Sigma-Aldrich
L-Ascorbic acid, tested according to Ph. Eur.
Pricing and availability is not currently available.
Sigma-Aldrich
L-Ascorbic acid, BioUltra, ≥99.5% (RT)
Pricing and availability is not currently available.
Supelco
Ethylene glycol, analytical standard
Pricing and availability is not currently available.
Supelco
L-Ascorbic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Pricing and availability is not currently available.
Sigma-Aldrich
Ethylene glycol, ReagentPlus®, ≥99%
Pricing and availability is not currently available.
Sigma-Aldrich
Ethylene glycol, spectrophotometric grade, ≥99%
Pricing and availability is not currently available.
Supelco
Sodium Citrate, Pharmaceutical Secondary Standard; Certified Reference Material
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Ethylene glycol, BioUltra, ≥99.5% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Ethylene glycol, anhydrous, 99.8%
SKU
Pack Size
Availability
Price
Quantity