Skip to Content
MilliporeSigma
  • First D1-like receptor PET imaging of the rat and primate kidney: implications for human disease monitoring.

First D1-like receptor PET imaging of the rat and primate kidney: implications for human disease monitoring.

American journal of physiology. Renal physiology (2014-05-09)
Michael L Granda, Frederick A Schroeder, Ronald H J Borra, Nathan Schauer, Ehimen Aisaborhale, Alexander R Guimaraes, Jacob M Hooker
ABSTRACT

The intrarenal dopamine system is important for signaling and natriuresis, and significant dysfunction is associated with hypertension and kidney disease in ex vivo studies. Dopamine receptors also modulate and are modulated by the renin-angiotensin-aldosterone system. Here, we show the first in vivo measurement of D1-like receptors in the renal cortex of Sprague-Dawley rat and Papio anubis baboon using [(11)C]NNC 112, a positron emission tomography radioligand for D1-like receptors. In addition, we show a D1-like binding potential response to angiotensin II blockade in rats using losartan. Demonstration of self-saturable binding in the rat as well as specific and saturable binding in Papio anubis validate the use of [(11)C]NNC 112 in the first in vivo measurement of renal dopamine D1-like receptors. Furthermore, [(11)C]NNC 112 is a radioligand tool already validated for use in probing human central nervous system (CNS) D1-like receptors. Our work demonstrates specific and saturable non-CNS binding in higher animals and the ability to quantify physiological response to drug treatment and provides a clear path to extend use of [(11)C]NNC 112 to study renal dopamine in humans.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Iodomethane, contains copper as stabilizer, ReagentPlus®, 99%
Sigma-Aldrich
Iodomethane, contains copper as stabilizer, ReagentPlus®, 99.5%
Sigma-Aldrich
Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)
Sigma-Aldrich
Iodomethane-12C, ≥99.9 atom % 12C, ≥99% (CP), contains copper as stabilizer
Supelco
Ethanol solution, certified reference material, 2000 μg/mL in methanol
Supelco
Dopamine hydrochloride, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Dehydrated Alcohol, Pharmaceutical Secondary Standard; Certified Reference Material
Dopamine hydrochloride, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Iodomethane, SAJ special grade, ≥99.5%
Sigma-Aldrich
Ethanol, JIS special grade, 94.8-95.8%
USP
Dehydrated Alcohol, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Dopamine hydrochloride
Sigma-Aldrich
Iodomethane, purum, ≥99.0% (GC)
Sigma-Aldrich
Ethanol, 94.8-95.8%
Sigma-Aldrich
Ethyl alcohol, Pure 190 proof, for molecular biology
Sigma-Aldrich
Ethanol, ≥99.5%, suitable for HPLC
Sigma-Aldrich
Ethanol, JIS 300, ≥99.5%, for residue analysis
Sigma-Aldrich
Dimethyl sulfoxide, SAJ first grade, ≥99.0%
Sigma-Aldrich
Ethanol, JIS 1000, ≥99.5%, for residue analysis
Sigma-Aldrich
Ethanol, ≥99.5%, suitable for absorption spectrum analysis
Sigma-Aldrich
Ethanol, ≥99.5%
Sigma-Aldrich
Dimethyl sulfoxide solution, 50 wt. % in H2O
Sigma-Aldrich
Potassium carbonate, anhydrous, powder, 99.99% trace metals basis
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.5%
Sigma-Aldrich
8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt, suitable for fluorescence, ≥90% (HPCE)
Sigma-Aldrich
Potassium carbonate, BioUltra, anhydrous, ≥99.0% (T)
Sigma-Aldrich
Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
Potassium carbonate, 99.995% trace metals basis
Sigma-Aldrich
Iodomethane solution, 2.0 M in tert-butyl methyl ether, contains copper as stabilizer
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%