- Artificial reductant enhancement of the Lowry method for protein determination.
Artificial reductant enhancement of the Lowry method for protein determination.
Analytical biochemistry (1986-06-01)
E Larson, B Howlett, A Jagendorf
PMID3728976
ABSTRACT
Addition of dithiothreitol in the Lowry procedure 3 min after adding the Folin-Ciocalteau reagent produces immediate color development, with 35 to 60% greater absorbance per mass of protein used.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Folin & Ciocalteu′s phenol reagent, suitable for determination of total protein by Lowry method, 1.9-2.1 N