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  • Purification of quadrol for microsequencing in the spinning-cup sequenator.

Purification of quadrol for microsequencing in the spinning-cup sequenator.

Analytical biochemistry (1984-04-01)
G S Begg, B H Leslie, F J Morgan
ABSTRACT

Treatment of Quadrol buffer with isothiocyanatophenylthiocarbamylaminoethylaminopropyl glass (DITC glass) substantially reduces the impurities observed when this buffer is used with the spinning cup sequenator. Use of DITC glass-treated Quadrol buffer permits identification of PTH-amino acids from protein degradations down to 100 pmol.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
N,N,N′,N′-Tetrakis(2-Hydroxypropyl)ethylenediamine, 98%