Skip to Content
MilliporeSigma
  • Defining the role of MRP-mediated efflux and glutathione in detoxification of oxaliplatin.

Defining the role of MRP-mediated efflux and glutathione in detoxification of oxaliplatin.

Die Pharmazie (2013-08-09)
C Mohn, H-G Häcker, R A Hilger, M Gütschow, U Jaehde
ABSTRACT

Albeit platinum complexes are widely used in cancer chemotherapy, their cellular processing has not been completely elucidated so far. In this study the effects of modulating multidrug resistance-associated protein (MRP)-mediated efflux and glutathione (GSH) depletion on the cytotoxicity of oxaliplatin were assessed in a human ileocecal colorectal adenocarcinoma cell line and its oxaliplatin-resistant variant. Upon oxaliplatin exposure, DNA platination was elevated by co-incubation with Gü83, a MRP1 and MRP2 inhibitor, but cytotoxicity was not increased. Addition of oxaliplatin did not alter the cellular GSH content. Following GSH depletion, platinum accumulation was unchanged but cytotoxicity was increased in oxaliplatin-sensitive cells. In conclusion, modulation of MRP-mediated efflux did not affect oxaliplatin cytotoxicity in the investigated cell lines. Intracellular GSH depletion seems to sensitize the cells but does not overcome resistance.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Platinum black, black, powder, ≤20 μm, ≥99.95% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, wire, diam. 0.127 mm, 99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum black, low bulk density, ≥99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, gauze, 100 mesh, 99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum black, fuel cell grade, ≥99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, shot, ≤3 mm, ≥99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, wire, diam. 0.25 mm, 99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, foil, thickness 0.127 mm, 99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, wire, diam. 0.076 mm, ≥99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, foil, thickness 0.025 mm, 99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, wire, diam. 2.0 mm, 99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, foil, thickness 0.25 mm, 99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, foil, thickness 0.1 mm, 99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Oxaliplatin, powder
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, powder, 99.995% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, foil, thickness 0.05 mm, ≥99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, powder (coarse), 99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, foil, thickness 1.0 mm, 99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, wire, diam. 0.25 mm, thermocouple grade
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, foil, thickness 0.025 mm, 99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, wire, diam. 1.5 mm, ≥99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, foil, thickness 0.05 mm, 99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, wire, diam. 0.5 mm, 99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, foil, thickness 0.125-0.135 mm, 99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, sponge, ≥99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, wire, diam. 0.20 mm, ≥99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, wire, diam. 0.10 mm, 99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, evaporation slug, diam. × L 0.6 cm × 1.2 cm, 99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, wire, diam. 0.5 mm, 99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Platinum, gauze, 52 mesh, 99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity