- Mucor hiemalis endo-beta-N-acetylglucosaminidase can transglycosylate a bisecting hybrid-type oligosaccharide from an ovalbumin glycopeptide.
Mucor hiemalis endo-beta-N-acetylglucosaminidase can transglycosylate a bisecting hybrid-type oligosaccharide from an ovalbumin glycopeptide.
Carbohydrate research (2004-10-13)
Kenji Osumi, Yoshitaka Makino, Eri Akaike, Takashi Yamanoi, Mamoru Mizuno, Midori Noguchi, Toshiyuki Inazu, Kenji Yamamoto, Kiyotaka Fujita
PMID15476727
ABSTRACT
We found that the recombinant endo-beta-N-acetylglucosaminidase of Mucor hiemalis (Endo-M) expressed in Candida boidinii had the transglycosylation activity of transferring a bisecting hybrid-type oligosaccharide from an ovalbumin glycopeptide to the acceptor (p-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside) in a good yield of 43%.