Skip to Content
MilliporeSigma
  • A comparison of fluorographic methods for the detection of 35S-labeled proteins in polyacrylamide gels.

A comparison of fluorographic methods for the detection of 35S-labeled proteins in polyacrylamide gels.

Analytical biochemistry (1988-09-01)
G G Perng, R D Rulli, D L Wilson, G W Perry
ABSTRACT

Eight different methods of fluorographic enhancement of sensitivity to 35S decay after gel electrophoresis were compared. Using Kodak X-Omat AR X-ray film, we found that some fluors were about equivalent to 2,5-diphenyloxazole/dimethyl sulfoxide embedding, whereas several other fluors were not quite as effective, but still were significantly more sensitive than control autoradiography. The most sensitive procedures can yield a detectable darkening of film with less than 1 dpm/mm2 of 35S after a 1-week exposure.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2,5-Diphenyloxazole, suitable for liquid scintillation spectrometry
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 kU
Please contact Customer Service for Availability
€153.00
25 kU
Please contact Customer Service for Availability
€520.00
Sigma-Aldrich
2,5-Diphenyloxazole, 99%, suitable for scintillation
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 kU
Please contact Customer Service for Availability
€153.00
25 kU
Please contact Customer Service for Availability
€520.00