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  • Fluorographic detection of [3H]thymidine-labeled deoxyribonucleic acid using agarose gels infused with 2,5-diphenyloxazole prior to electrophoresis.

Fluorographic detection of [3H]thymidine-labeled deoxyribonucleic acid using agarose gels infused with 2,5-diphenyloxazole prior to electrophoresis.

Analytical biochemistry (1985-02-15)
J W Ludlow, K C Yuen, R A Consigli
ABSTRACT

A fluorographic procedure for the detection of [3H]thymidine-labeled deoxyribonucleic acids electrophoresed in agarose gels was developed. 2,5-Diphenyloxazole (PPO) was added to the agarose solution before pouring of the gel for electrophoresis. This procedure did not interfere with the electrophoretic mobility of the DNA molecules. The radioactive detection efficiency was found to be improved over an existing procedure whereby the agarose gel was infused with PPO after electrophoresis with the aid of acetic acid.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2,5-Diphenyloxazole, suitable for liquid scintillation spectrometry
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500 g
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€104.00
1 kg
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€214.00
5 kg
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€445.00
Sigma-Aldrich
2,5-Diphenyloxazole, 99%, suitable for scintillation
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
Please contact Customer Service for Availability
€104.00
1 kg
Please contact Customer Service for Availability
€214.00
5 kg
Please contact Customer Service for Availability
€445.00