Skip to Content
MilliporeSigma
  • Effect of magnesium on gastrointestinal transit time in normal and diabetic rats: possible mechanism of action.

Effect of magnesium on gastrointestinal transit time in normal and diabetic rats: possible mechanism of action.

African journal of medicine and medical sciences (2013-05-16)
E O Adewoye, A O Ige
ABSTRACT

Many gastrointestinal complications in diabetes are connected to neurohumoral dysfunction resulting in abnormalities of intestinal motility, secretion and absorption. Minerals have been reported as essential cofactors for basic cellular reactions but there is dearth of information on effect of Magnesium on gastrointestinal transit time (GITT) and the mechanism of action. Sixty male albino Wistar rats (180 - 200g) were grouped into twelve of five animals each. Group 1 (control) received 0.2ml saline. Groups 2-6 were normal rats treated with magnesium sulphate (as magnesium) (500mg/kg), adrenaline (0.5mg/kg), magnesium (500mg/kg) and adrenaline (0.5mg/kg), prazosin (1mg/kg) and both magnesium (500mg/kg) and prazosin (1mg/kg) respectively. Groups 7 - 12 were diabetic rats treated as in groups 1- 6. Diabetes was induced intraperitoneally with alloxan (120mg/kg bwt). There was significant (p<0.05) reduction in GITT index in normal rats treated with magnesium, prazosin and combination of magnesium and prazosin compared with control. Treatment with adrenaline alone produced significant increase in GITT. However treatment with both magnesium and adrenaline produced significant reduction compared with control. This reduction in GITT was similar to that obtained in magnesium only and prazosin only treated groups. Diabetic groups showed significant reduction in GITT in all treated groups except the adrenaline only treated group which produced significant increase in GITT. The significant reduction in GITT produced by magnesium in both normal and diabetic animals was comparable to that produced by prazosin (an á-adrenoceptor antagonist) indicating that magnesium may be inhibiting gastrointestinal smooth muscle contraction through á-adrenoceptor antagonist pathway.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Magnesium sulfate heptahydrate, BioUltra, ≥99.5% (KT)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Magnesium sulfate heptahydrate, BioXtra, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Magnesium sulfate heptahydrate, BioReagent, for molecular biology, suitable for plant cell culture, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Adrenaline, European Pharmacopoeia (EP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Adrenaline with impurity F, European Pharmacopoeia (EP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Magnesium sulfate heptahydrate, puriss. p.a., ACS reagent, ≥99.0% (KT)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Magnesium sulfate heptahydrate, ACS reagent, ≥98%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Magnesium sulfate heptahydrate, ReagentPlus®, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Magnesium sulfate heptahydrate, 99.5-100.5% (calc. to the dried substance), meets analytical specification of Ph. Eur., BP,USP, FCC
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
(−)-Epinephrine
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Magnesium sulfate, BioReagent, suitable for cell culture, suitable for insect cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Magnesium sulfate solution, for molecular biology, 1.00 M±0.04 M
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Magnesium sulfate, ≥99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Prazosin hydrochloride, ≥99.0% (HPLC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Magnesium sulfate solution, BioUltra, for molecular biology
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Magnesium sulfate, anhydrous, ReagentPlus®, ≥99.5%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Magnesium sulfate, anhydrous, reagent grade, ≥97%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Magnesium sulfate, puriss. p.a., drying agent, anhydrous, ≥98.0% (KT), powder (very fine)
SKU
Pack Size
Availability
Price
Quantity