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  • Catalytic biofilms on structured packing for the production of glycolic acid.

Catalytic biofilms on structured packing for the production of glycolic acid.

Journal of microbiology and biotechnology (2013-02-16)
Xuan Zhong Li, Bernhard Hauer, Bettina Rosche
ABSTRACT

While structured packing modules are known to be efficient for surface wetting and gas-liquid exchange in abiotic surface catalysis, this model study explores structured packing as a growth surface for catalytic biofilms. Microbial biofilms have been proposed as self-immobilized and self-regenerating catalysts for the production of chemicals. A concern is that the complex and dynamic nature of biofilms may cause fluctuations in their catalytic performance over time or may affect process reproducibility. An aerated continuous trickle-bed biofilm reactor system was designed with a 3 L structured packing, liquid recycling and pH control. Pseudomonas diminuta established a biofilm on the stainless steel structured packing with a specific surface area of 500 m2 m-3 and catalyzed the oxidation of ethylene glycol to glycolic acid for over two months of continuous operation. A steady-state productivity of up to 1.6 gl-1h-1 was achieved at a dilution rate of 0.33 h-1. Process reproducibility between three independent runs was excellent, despite process interruptions and activity variations in cultures grown from biofilm effluent cells. The results demonstrate the robustness of a catalytic biofilm on structured packing, despite its dynamic nature. Implementation is recommended for whole-cell processes that require efficient gas-liquid exchange, catalyst retention for continuous operation, or improved catalyst stability.

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Sigma-Aldrich
Glycolic acid, ReagentPlus®, 99%
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100 mg
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Glycolic acid solution, high purity, 70 wt. % in H2O
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100 mg
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Glycolic acid solution, technical grade, 70 wt. % in H2O
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100 mg
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Ethylene glycol, JIS special grade, ≥99.5%
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100 mg
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Glycolic acid, SAJ special grade, ≥98.0%
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100 mg
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Ethylene glycol, SAJ first grade, ≥99.0%
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100 mg
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Glycolic acid solution, CP, 70% in H2O
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100 mg
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Supelco
Ethylene glycol, Pharmaceutical Secondary Standard; Certified Reference Material
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100 mg
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100 mg
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100 mg
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100 mg
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Ethylene glycol solution, NMR reference standard, 80% in DMSO-d6 (99.9 atom % D), NMR tube size 5 mm × 8 in.
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Glycolic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
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100 mg
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Ethylene glycol, BioUltra, ≥99.5% (GC)
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100 mg
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100 mg
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