- A new technique for detecting oxytocinase activity in electrophoresis gels.
A new technique for detecting oxytocinase activity in electrophoresis gels.
Electrophoresis (1992-06-01)
A C Roy, N Saha, S M Tan, F Z Kamarul, S S Ratnam
PMID1505501
ABSTRACT
A sensitive staining method for the detection of oxytocinase (EC 3.4.11.3) activity in electrophoresis gels has been described. The method is based on the enzymatic release of p-nitroaniline (PNA) from two specific synthetic oxytocinase substrates, S-benzyl-L-cysteine-p-nitroanilide (BCN) and L-leucine-p-nitroanilide (LN), respectively. The PNA is then diazotized with sodium nitrite and subsequently coupled to a chromogen, N-(1-naphthyl)-ethylenediamine dihydrochloride (NED) to produce a deep pink/magenta colored azo-dye at the site of oxytocinase activity.
MATERIALS
Product Number
Brand
Product Description
Supelco
N-(1-Naphthyl)ethylenediamine dihydrochloride monomethanolate, for spectrophotometric det. of nitrate and nitrite, ≥99.0%
Supelco
N-(1-Naphthyl)ethylenediamine dihydrochloride, for determination of sulfonamide and nitrite, ACS reagent, ≥98%