Skip to Content
MilliporeSigma
  • Does platelet-rich plasma freeze-thawing influence growth factor release and their effects on chondrocytes and synoviocytes?

Does platelet-rich plasma freeze-thawing influence growth factor release and their effects on chondrocytes and synoviocytes?

BioMed research international (2014-08-20)
Alice Roffi, Giuseppe Filardo, Elisa Assirelli, Carola Cavallo, Annarita Cenacchi, Andrea Facchini, Brunella Grigolo, Elizaveta Kon, Erminia Mariani, Loredana Pratelli, Lia Pulsatelli, Maurilio Marcacci
ABSTRACT

PRP cryopreservation remains a controversial point. Our purpose was to investigate the effect of freezing/thawing on PRP molecule release, and its effects on the metabolism of chondrocytes and synoviocytes. PRP was prepared from 10 volunteers, and a half volume underwent one freezing/thawing cycle. IL-1β, HGF, PDGF AB/BB, TGF-β1, and VEGF were assayed 1 hour and 7 days after activation. Culture media of chondrocytes and synoviocytes were supplemented with fresh or frozen PRP, and, at 7 days, proliferation, gene expression, and secreted proteins levels were evaluated. Results showed that in the freeze-thawed PRP the immediate and delayed molecule releases were similar or slightly lower than those in fresh PRP. TGF-β1 and PDGF AB/BB concentrations were significantly reduced after freezing both at 1 hour and at 7 days, whereas HGF concentration was significantly lower in frozen PRP at 7 days. In fresh PRP IL-1β and HGF concentrations underwent a significant further increase after 7 days. Similar gene expression was found in chondrocytes cultured with both PRPs, whereas in synoviocytes HGF gene expression was higher in frozen PRP. PRP cryopreservation is a safe procedure, which sufficiently preserves PRP quality and its ability to induce proliferation and the production of ECM components in chondrocytes and synoviocytes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Esculin hydrate, ≥98.0% (HPLC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 g
Please contact Customer Service for Availability
€54.60
1 kg
Please contact Customer Service for Availability
€144.00
5 kg
Please contact Customer Service for Availability
€511.00