Disruption of autophagy by increased 5-HT alters gut microbiota and enhances susceptibility to experimental colitis and Crohn's disease.

Disruption of autophagy by increased 5-HT alters gut microbiota and enhances susceptibility to experimental colitis and Crohn's disease.

Science advances (2021-11-06)

Sabah Haq, Huaqing Wang, Jensine Grondin, Suhrid Banskota, John K Marshall, Irfan I Khan, Usha Chauhan, Francine Cote, Yun Han Kwon, Dana Philpott, John H Brumell, Michael Surette, Gregory R Steinberg, Waliul I Khan

PMID34739317

ABSTRACT

[Figure: see text].

MATERIALS

Product Number

Brand

Product Description

H9772

Sigma-Aldrich

5-Hydroxy-L-tryptophan, powder

P8340

Sigma-Aldrich

Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

37094

Supelco

Rapamycin, VETRANAL^®, analytical standard

H9523

Sigma-Aldrich

Serotonin hydrochloride, powder

L1668

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean), Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture