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  • Syntheses of immunomodulating androstanes and stigmastanes: comparison of their TNF-alpha inhibitory activity.

Syntheses of immunomodulating androstanes and stigmastanes: comparison of their TNF-alpha inhibitory activity.

Bioorganic & medicinal chemistry (2007-09-26)
Javier A Ramírez, Andrea C Bruttomesso, Flavia M Michelini, Sofía L Acebedo, Laura E Alché, Lydia R Galagovsky
ABSTRACT

In a previous work our group showed that some synthetic stigmastanes may play a role in immune-mediated inflammation. In this paper we report the syntheses of a series of new steroidal compounds derived from dehydroepiandrosterone and stigmasterol, and the evaluation of their in vitro inhibitory activity of the TNF-alpha production by macrophages. A preliminary qualitative structure-activity relationship was established.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
trans-Dehydroandrosterone, ≥99%
Supelco
Dexamethasone, VETRANAL®, analytical standard
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Dexamethasone, powder, BioReagent, suitable for cell culture, ≥97%
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Dexamethasone, meets USP testing specifications
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Dexamethasone, ≥98% (HPLC), powder
Sigma-Aldrich
Dexamethasone, powder, γ-irradiated, BioXtra, suitable for cell culture, ≥80% (HPLC)
Sigma-Aldrich
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