53542-U

Ascentis^® Express Peptide 160 Å ES-C18 (2.7 μm) HPLC Columns

L × I.D. 5 mm × 4.6 mm, HPLC Guard Cartridge, pkg of 3 ea

• Synonym(s): Superficially Porous Wide Pore C18 Guard Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Ascentis^® Express Peptide ES-C18, 2.7 μm Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 4.6 mm, pkg of 3 ea
• material: stainless steel column
• agency: suitable for USP L1
• product line: Ascentis^®
• feature: endcapped: no
• packaging: pkg of 3 ea
• extent of labeling: 4.6% carbon loading
• technique(s): HPLC: suitable; LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable
• L × I.D.: 5 mm × 4.6 mm
• surface area: 90 m²/g
• matrix: superficially porous particle
• matrix active group: C18 (octadecyl) phase
• particle size: 2.7 μm
• pore size: 160 Å
• operating pH: 1-9
• separation technique: reversed phase

Description

General description

Ascentis Express Guard Columns provide physical (filtration) and chemical protection for costly analytical columns without compromising the very high performance of Ascentis Express columns. These Ascentis Express guard columns are capable of continuous use at pressures up to 9000 psi (600 bar) with only hand-tightening. Guard cartridges are easily replaced without removing the guard column holder from the flow path. The cartridges are packed with Ascentis Express Fused-Core^®particles. Order guard column holder (53500-U) separately.

Application

• Proteotypic Peptide Identification in Biosynthetic Insulin: Utilizes Ascentis^® Express Peptide ES-C18, 2.7 μm Guard Cartridge in multiplexed targeted mass spectrometry to identify proteotypic tryptic peptides of recombinant human insulin and biosynthetic insulin analogues, demonstrating high resolution and precision in peptide mapping essential for biopharmaceutical analysis (Qasem et al., 2019).


• Preclinical Pharmacokinetic Study of Thymopentin: Employs the Ascentis^® Express Peptide ES-C18, 2.7 μm Guard Cartridge for the high-resolution separation and quantitative determination of thymopentin in beagle dog blood, highlighting its application in pharmacokinetic studies and drug development (Shi et al., 2015).


• Cytochrome P450 and UDP-Glucuronosyltransferases Quantification: Features the use of Ascentis^® Express Peptide ES-C18, 2.7 μm Guard Cartridge in a targeted proteomics approach for the absolute quantification of clinically relevant enzymes, offering insights into protein expression levels critical for drug metabolism and pharmacogenomics studies (Gröer et al., 2014).


• Carotenoid Analysis in Superficially Porous Phases: Demonstrates the capability of the Ascentis^® Express Peptide ES-C18, 2.7 μm Guard Cartridge in extending the carotenoid test to new stationary phases, underlining its versatility and effectiveness in analytical chromatography for complex natural products (Lesellier, 2012).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.