GE17-5481-51

HiTrap^® Capto^™ Core 700

Cytiva 17-5481-51, pack of 5 × 1 mL

• Synonym(s): Capto Core 700 Column
• UNSPSC Code: 23151817
• NACRES: NA.56

Properties

• ligand: octylamine
• packaging: pack of 5 × 1 mL
• manufacturer/tradename: Cytiva 17-5481-51
• parameter: 5 bar
• column I.D.: 7 mm
• matrix: highly cross-linked agarose
• avg. part. size: 75 (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 3-14(pH interval where the medium can be subjected to, for cleaning- or sanitization-in-place.)
• working range: 2-14(pH interval where the medium can be operated without significant change in function.)
• capacity: ~13 mg binding capacity (ovalbumin)
• suitability: suitable for bioprocess medium

Description

General description

Dynamic binding capacities were measured at 10% breakthrough with a residence time of 3 min (1.6 ml/min = 200 cm/h) in HiScreen columns. Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH 7.5.

HiTrap Capto Core 700 is prepacked with Capto Core 700, a multimodal BioProcess resin for intermediate purification and polishing of viruses and other large biomolecules. Capto Core 700 is a novel, layered bead chromatography resin consisting of a porous outer layer and ligand-activated core. The octylamine ligands found in the core of the beads are multimodal, being both hydrophobic and positively charged. These internalized ligands bind impurities strongly over a wide range of pH and salt concentration. The pores of the bead outer layer prevent large targets (Mr > 700 000) from binding to the ligands while smaller impurities can enter freely into the beads where they are captured.

Features and Benefits

• High capacity and productivity in flowthrough mode.
• Prepacked HiTrap columns for easy screening and convenient process development, as well as small scale purification.
• Wide operational window of pH and conductivity.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Capto is a trademark of Cytiva

HiTrap is a registered trademark of Cytiva

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids