You can deproteinize by using barium hydroxide, B4059, and zinc sulfate, Z2876, by adding 0.2 ml of sample to 1.8 ml of water, swirl and mix, then 1.0 ml of barium hydroxide, swirl and mix, then 1.0 ml of zinc sulfate, swirl and mix thoroughly by shaking, and then filter or centrifuge to obtain clear filtrate.
GAHK20
Glucose (HK) Assay Kit
sufficient for 20 assays
Synonym(s):
Glucose Quantification Kit
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$78.60
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1 KIT
$78.60
About This Item
UNSPSC Code:
12164500
NACRES:
NA.84
Recommended Products
usage
sufficient for 20 assays
Quality Level
analyte chemical class(es)
sugars (glucose)
technique(s)
photometry: suitable
application(s)
food and beverages
general analytical
storage temp.
2-8°C
Application
This kit is for the quantitative, enzymatic determination of glucose in food and other material. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phospho-gluconate in the presence of oxidized nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to glucose concentration.
Kit Components Only
Product No.
Description
- glucose (HK) assay reagent 20 mL
- glucose standard .5 mL
related product
Storage Class
10 - Combustible liquids
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Kunst, A., et al.
Methods of Enzymatic Analysis, 163-172 (1984)
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If my sample for the Glucose (HK) Assay Kit, Product GAHK20, has a lot of protein, how do I deproteinize it?
1 answer-
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How do I degas a solution for use with Product GAHK20, Glucose (HK) Assay Kit?
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You can degas a solution by either putting it under a vacuum or by bubbling nitrogen through it.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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