F8931

Anti-Flotillin 1-CY3 antibody produced in rabbit

1.0-3.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-Flot1
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IF (d)
• Citations: 5

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, mouse, rat
• concentration: 1.0-3.0 mg/mL
• technique(s): direct immunofluorescence: 2-5 μg/mL using human HEK-293T or HeLa cells
• UniProt accession no.: O75955
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FLOT1(10211) ; mouse ... Flot1(14251) ; rat ... Flot1(64665)

Description

General description

Flotillins are lipid raft-associated proteins belonging to the integral membrane proteins. It is associated in different biological processes such as endocytosis, phagocytosis, neuronal regeneration, insulin signaling etc. It exhibits an evolutionarily conserved domain called the prohibitin homology (PHB) domain or SPFH domain (stomatin/prohibitin/flotillin/HflK/C domain). The lipid recognition motif present in the PHB domain, is responsible for targeting proteins to the plasma membrane. Flotillin protein mainly possess two forms, flotillin 1 and 2. They are also known as reggie-2 and 1, respectively.

Immunogen

synthetic peptide corresponding to amino acid residues 413-428 of mouse flotillin 1, conjugated to KLH. The corresponding sequence is identical in rat and differs by one amino acid in human.

Application

Anti-Flotillin 1-CY3^™ antibody is suitable for direct immunofluorescence at a concentration of 2-5μg/mL using human HEK-293T or HeLa cells

Biochem/physiol Actions

Flotillin 1 is directly connected to the plasma membrane. The association of flotillin 1 depends on the palmitoylation in a conserved Cys34 residue, whereas the association of flotillin 2 depends on both myristolation and palmitoylation. Both the form possess a complementary tissue distribution. Flotillin 1 is mainly expressed in striated muscle tissues, adipose tissue and lung, whereas flotillin 2 is much widely found but absent from skeletal muscle. It has been reported that flotillin 1 act as a molecular link between lipid rafts of the plasma membrane and a multimeric signaling complex at the actin cytoskeleton.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Legal Information

Cy3 is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable