Skip to Content
MilliporeSigma
All Photos(1)

Documents

F5554

Sigma-Aldrich

Formalin, 10%, Neutral Buffered, Wintergreen Scented

histological fixative, pH 6.9-7.1

Synonym(s):

Formaldehyde, Methanal

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12171500
NACRES:
NA.47

shelf life

Expiry date on the label.

color

colorless

pH

6.90-7.10

application(s)

hematology
histology

Storage temp.

room temp

General description

10% Neutral buffered formalin (NBF) is the most commonly used fixative for light microscopy. 10% NBF is well suited for large throughput laboratories and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship.

Application

Formalin, 10%, Neutral Buffered, Wintergreen Scented has been used as a fixative in transformation and tumorigenicity assay and in immunohistostaining.
Intended for use as a general purpose histological fixative. Wintergreen oil has been added to the standard formulation to provide a wintergreen scent.

Pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Effects of formalin fixation on tissue optical
M F G Wood
Physics in Medicine and Biology, 56, V115-V115 (2011)
Toward deciphering proteomes of formalin-fixed paraffin-embedded (FFPE) tissues
Magdeldin, et al.
Proteomics, 12(7), 1045-1058 (2012)
Flow cytometric determination of stem/progenitor content in epithelial tissues: an example from nonsmall lung cancer and normal lung
Donnenberg VS, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 83(1), 141-149 (2013)
A method to estimate the formaldehyde concentration in fixative solutions
Simos, P
Pathology, 43, 394-394 (2001)
Kelly B Engel et al.
Archives of pathology & laboratory medicine, 135(5), 537-543 (2011-04-30)
While formalin fixation and paraffin embedding has become a universal mechanism of tissue preservation and a gold standard for immunohistochemistry, fixation and processing variables that may confound assay effectiveness have received little attention from the scientific community. To identify discrete

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service