E3906

Anti-Early Endosomal Antigen 1 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-EEA1, Anti-Endosome-associated Protein p162, Anti-Zinc Finger FYVE Domain-containing Protein 2
• MDL number: MFCD01633310
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~160 kDa
• species reactivity: mouse, rat, human
• concentration: ~1 mg/mL
• technique(s): indirect immunofluorescence: 5-10 μg/mL using rat NRK cells; western blot (chemiluminescent): 0.1-0.2 μg/mL using whole extract of human HeLa cells; western blot (chemiluminescent): 0.2-0.4 μg/mL using whole extract of mouse NIH3T3 cells
• UniProt accession no.: Q15075
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EEA1(8411) ; mouse ... Eea1(216238) ; rat ... Eea1(314764)

Description

General description

Anti-Early Endosomal Antigen 1 (C-terminal), is produced in rabbit using a synthetic peptide corresponding to amino acid residues 1391-1410 of human EEA1, conjugated to keyhole limpet hemocyanin (KLH), as immunogen. Early Endosomal Antigen 1 (EEA1) is a 162 kDa autoantigen, which encodes for around 1400 amino acid proteins. EEA1 is a dimer, which comprises extensive coiled-coil regions. At its C-terminus, it contains a cysteine-rich zinc-finger-like domain named FYVE domain. The gene Early Endosome Antigen 1 (EEA1) encodes for around 1400 amino acid proteins

Immunogen

synthetic peptide corresponding to amino acid residues 1391-1410 of human EEA1, conjugated to KLH. The corresponding sequence is identical in mouse.

Application

Anti-Early Endosomal Antigen 1 (C-terminal) antibody produced in rabbit has been used in:

• fluorescent staining
• confocal laser scanning microscopy
• immunoprecipitation
• indirect immunofluorescence microscopy
• fluorescence microscopy
• immunoblotting
• immunofluorescence.

Biochem/physiol Actions

Anti- Early Endosomal Antigen 1 (EEA1) may be used as an early endosome marker. The binding of EEA1 to phosphatidylinositol 3-phosphate (PtdIns3P) and Rab5-GTP is essential for the localization and function of EEA1 in endocytic membrane fusion. It is associated with subacute systemic lupus erythematosus that specifically localized to early endosomes.

The gene Early Endosome Antigen 1 (EEA1) encodes for around 1400 amino acid proteins. It is a peripheral membrane protein associated with the cytoplasmic side of early endosomes. EEA1 controls vesicle fusion during endocytosis. In neurons, it is involved in recycling of synaptic vesicles and neurotransmitter receptors. Patients with neurological deficits develop EEA1 autoantibodies. Mutation in EEA1 gene leads to susceptibility to diabetes in the Japanese population.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)