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Key Documents

UM-UC-6

8090503, human bladder, Epithelial

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About This Item

UNSPSC Code:
41106514

product name

UM-UC-6, 08090503

biological source

human bladder

growth mode

Adherent

karyotype

Modal number of chromosomes is 51. See reference.

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human transitional cell carcinoma of the bladder

Cell Line Description

UM-UC-6 was obtained by transurethral resection of a bladder transitional cell carcinoma found in a male patient. UM-UC-6 was found to produce tumours in athymic nude mice.

Application

Drug resistance in bladder neoplasms

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11
D13S317: 13,14
D16S539: 9,13
D5S818: 11,13
D7S820: 8,10
THO1: 7
TPOX: 9,11
vWA: 18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. Doubling time approximately 38 hours.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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