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CASP3F

Sigma-Aldrich

Caspase 3 Assay Kit, Fluorimetric

Synonym(s):

Caspase-3 Activity Fluorometric Kit

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About This Item

EC Number:
247-454-8
UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 1,000 multiwell tests
 sufficient for 100 standard tests (2 ml)

Quality Level

200

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CASP3(836)

General description

The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).

Application

The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.

Unit Definition

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.

Kit Components Only

Product No.
Description
  • Water (17-18 megaOhm) 125 mL

Kit Components Also Available Separately

Product No.
Description
SDS
  • A1086Ac-DEVD-AMC substrate 2.5 mgSDS
  • A0835Ac-DEVD-CHO inhibitor .5 mgSDS
  • A98917-Amino-4-methylcoumarin standard 1 mgSDS

related product

Product No.
Description
Pricing

pictograms

Exclamation mark
GHS07

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Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

Certificates of Analysis (COA)

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Zinc octa-n-alkyl phthalocyanines in photodynamic therapy: photophysical properties, accumulation and apoptosis in cell cultures, studies in erythrocytes and topical application to Balb/c mice skin
Kaestner,L et al.
Photochemistry and Photobiology, 2, 660-667 (2003)
V A Smith et al.
The British journal of ophthalmology, 88(5), 619-625 (2004-04-20)
Doxycycline is a broad spectrum antibiotic that chelates metal ions and is frequently used as part of the treatment of ocular surface diseases. Its therapeutic value has been ascribed to an ability to inhibit matrix metalloproteinase (MMP) activity and both
Radharaman Ray et al.
Drug and chemical toxicology, 28(1), 105-116 (2005-02-22)
Sulfur mustard [SM; bis-(2-chloroethyl) sulfide], which causes skin blistering or vesication [(1991). Histo- and cytopathology of acute epithelial lesions. In: Papirmeister, B., Feister, A. J., Robinson, S. I., Ford, R. D., eds. Medical Defense Against Mustard Gas: Toxic Mechanisms and
Sau Wai Hung et al.
PloS one, 8(8), e71196-e71196 (2013-08-14)
We evaluated the potential of an investigational histone methylation reversal agent, 3-deazaneplanocin A (DZNep), in improving the chemosensitivity of pancreatic cancer to nucleoside analogs (i.e., gemcitabine). DZNep brought delayed but selective cytotoxicity to pancreatic cancer cells without affecting normal human
N M Martins et al.
Journal of applied toxicology : JAT, 28(3), 337-344 (2007-07-03)
Cisplatin is a potent and widely used chemotherapeutic agent. Nephrotoxicity induced by this drug has been well documented. However, very little information is available on cisplatin-induced hepatotoxicity and its underlying mechanism remains unclear. High doses of cisplatin have been known
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