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Key Documents

A2289

Sigma-Aldrich

Albumin, Tetramethylrhodamine isothiocyanate bovine

lyophilized powder

Synonym(s):

TRITC-albumin

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About This Item

CAS Number:
9048-46-8
MDL number:
MFCD00130416
UNSPSC Code:
12352202
NACRES:
NA.25

biological source

bovine

Quality Level

200

assay

≥90% (Lowry)

form

lyophilized powder

extent of labeling

≥0.5 mol TRITC per mol albumin

technique(s)

cell culture | mammalian: suitable

impurities

salt, free

UniProt accession no.

P02769

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

General description

Tetramethylrhodamine isothiocyanate (TRITC) is stable and less sensitive to light compared to fluorescein isothiocyanate (FITC). The conjugate of albumin with TRITC is effective for following microcirculation in vivo microscopy. Albumin-TRITC aids tracking of calcium phosphate nanoparticle bound DNA in human umbilical vein endothelial cells (T-HUVEC).

Application

Albumin, Tetramethylrhodamine isothiocyanate has been used:
  • in photoinduced release assay of polyethylene beads
  • for passivating poly(methyl methacrylate) (PMMA) layer prior to immunoglobulin G coating
  • in fluorescent correlation spectroscopy

Red-fluorescing TRITC-albumin was used to track the uptake of calcium phosphate/DNA nanoparticles during cell transfection. The dye-linked albumin is incorporated into the nanoparticles. Efficiency of transfection could be followed by fluorescence microscopy.

Preparation Note

Prepared from crystallized and lyophilized albumin (A4378).

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificates of Analysis (COA)

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Photoresponsive Aqueous Dissolution of Poly (N-Isopropylacrylamide) Functionalized with o-Nitrobenzaldehyde through Phase Transition
Sumaru K, et al.
Biomacromolecules, 19(7), 2913?2922-2913?2922 (2018)
Tracking the pathway of calcium phosphate/DNA nanoparticles during cell transfection by incorporation of red-fluorescing tetramethylrhodamine isothiocyanate-bovine serum albumin into these nanoparticles
Sokolova V, et al.
Journal of Biological Inorganic Chemistry, 12(2), 174-179 (2007)
Fluorescent Biorecognition of Gold Nanoparticle-IgG Conjugates Self-Assembled on E-Beam Patterns
Powell T and Yoon JY
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Correct diffusion coefficients of proteins in fluorescence correlation spectroscopy. Application to tubulin oligomers induced by Mg2+ and Paclitaxel
Krouglova T, et al.
Biophysical Journal, 87(4), 2635-2646 (2004)
Evaluation of fluorescent plasma markers for in vivo microscopy of the microcirculation
Reeves KJ, et al.
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