SRP0134

EZH2/EED/SUZ12/RbAp48/AEBP2 human

recombinant, expressed in baculovirus infected insect cells

• Synonym(s): ENX-1, EZH2, Enhancer of zeste homolog 2, KMT6A, Lysine N-methyltransferase 6A
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• assay: ≥80% (SDS-PAGE)
• form: aqueous solution
• mol wt: 48 kDa (RbAp48); 51 kDa (EED); 53 kDa (AEBP2); 86 kDa (EZH2); 87 kDa (SUZ12)
• packaging: pkg of 50 μg
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_003797 (EED); NM_004456 (EZH2); NM_005610 (RbAP48); NM_015355 (SUZ12); NM_153207 (AEBP2)
• UniProt accession no.: Q15910
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... EZH2(2146)

Description

General description

EZH2, EED, SUZ12, RbAp48 and AEBP2 are components of the polycomb repressive complex 2 (PRC2). The highly conserved complex methylates lysine 27 of histone H3. It also has a role in gene silencing and transcription repression.

Application

EZH2/EED/SUZ12/RbAp48/AEBP2 human is useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable