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R150-05N

Sigma-Aldrich

Rat Skeletal Muscle Cells: RSkMC, neonatal

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

rat skeletal muscle (normal limb)

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 42

morphology

Skeletal muscle

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

muscular dystrophy

shipped in

dry ice

storage temp.

−196°C

General description

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Rat Skeletal Muscle Cells (RSkMC) provide a useful system to study many aspects of muscular function and disease and can serve as an intermediate model of human diseases. Skeletal Muscle Cells also play an instrumental role in the glucose metabolism and diabetes.

Cell Line Origin

Muscle

Application

lipid and glucose metabolism, exercise, insulin action, cell signaling

Components

Rat Skeletal Muscle Cell Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • 1st passage, >500,000 cells in Rat Skeletal Muscle Cell Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 10 doublings

Subculture Routine

Please refer to the RSkMC Culture Protocol.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Marta Milewska et al.
Journal of cellular physiology, 234(11), 19675-19690 (2019-04-05)
Interleukin (IL)-8 is released both in visceral adipose tissue and in contracting skeletal muscles. In this study, we examined cellular pathways associated with muscle hypertrophy, chosen on the basis of microRNA profiling, in differentiating rat primary skeletal muscle cells (RSkMC)

Protocols

Rat Skeletal Muscle Cells (RSkMC) Culture Protocol

Technical information for working with rat skeletal muscle cells including thawing, subculturing and cryopreservation

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