Skip to Content
MilliporeSigma
All Photos(1)

Documents

N1531

Sigma-Aldrich

Denaturation Solution

for Northern and Southern blotting

Synonym(s):

Denaturation Solution For Blotting Technique, Denaturation Solution for Neutral Southern Transfer

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

for molecular biology

form

solution

storage temp.

room temp

General description

Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.

application

Denaturation Solution is suitable for denaturing dsDNA before the transfer to membranes during Northern and Southern blotting procedures.

Components

The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.

Other Notes

0.5 N NaOH, 1.5 M NaCl

related product

Product No.
Description
Pricing

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H290,H314

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

José Manuel Pérez-Pérez et al.
Plant physiology, 134(1), 101-117 (2004-01-20)
The dwarf ucu (ultracurvata) mutants of Arabidopsis display vegetative leaves that are spirally rolled downwards and show reduced expansion along the longitudinal axis. We have previously determined that the UCU1 gene encodes a SHAGGY/GSK3-like kinase that participates in the signaling
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Ausubel, F.M., et al.
Short Protocols in Molecular Biology (1995)

Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service