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MAK180

Sigma-Aldrich

High Sensitivity Fructose Assay Kit

Sufficient for 100 Fluorometric tests

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About This Item

EC Number:
UNSPSC Code:
12352200
NACRES:
NA.84

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

storage temp.

−20°C

General description

Fructose is a monosaccharide naturally found in honey and fruits. Several studies have linked fructose to the development of the metabolic syndrome particularly through promoting insulin resistance in the liver. It has also been implicated in the obesity epidemic. Studies in animals have revealed high fructose intake during pregnancy and lactation may lead to metabolic dysfunctions in the mother and the newborn. Fructose measurements can provide useful insights into metabolic and biochemical functions.

Fructose concentration is determined by a coupled enzyme assay, which results in a fluorometric (lex = 535/lem = 587 nm) product proportional to the fructose present.

Suitability

This kit is a highly sensitive assay for determining D-fructose levels (ranging from 20-100 pmole/well) in a variety of biological samples.

Principle

Fructose concentration is determined by a coupled enzyme assay, which results in a fluorometric (λex = 535/λem = 587 nm) product proportional to the fructose present.

Kit Components Only

Product No.
Description

  • Fructose Assay Buffer

  • Fructose Probe

  • Sample Cleanup Mix

  • Conversion Enzyme

  • Fructose Enzyme Mix

  • Fructose Substrate Mix

  • Fructose Standard, 100 mM

Storage Class

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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