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L9780

Sigma-Aldrich

Lipase from human pancreas

buffered aqueous solution, ≥250 units/mg protein (Lowry)

Synonym(s):

Triacylglycerol acylhydrolase, Triacylglycerol lipase

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204

form

buffered aqueous solution

specific activity

≥250 units/mg protein (Lowry)

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... PNLIP(5406)

General description

Pancreatic lipase fulfills a key function in dietary fat absorption by hydrolyzing triglycerides into diglycerides and subsequently into monoglycerides and free fatty acids.

Application

Lipase has been used in a study to assess the effects of acidification on human milk′s cellular and nutritional content. It has also been used in a study to investigate the effect of physical training on the adipose tissue of diet-induced obesity mice.

Biochem/physiol Actions

The hormone leptin reduces pancreatic secretion and expression of lipase and has been shown to directly inhibit its activity.
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Unit Definition

One unit will liberate 1.0 μmole of 2-monoglyceride from 1,2-diglyceride per min at pH 8.1 at 37 °C.

Physical form

Solution in 0.1 M Tris containing 0.1 M NaCl and serine protease inhibitor

Analysis Note

Protein determined by Lowry.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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The effects of acidification on human milk's cellular and nutritional content
Erickson, T., et al.
Journal of Perinatology : Official Journal of the California Perinatal Association, doi: 10-doi: 10 (2012)
J M de Farias et al.
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme, 45(3), 190-196 (2012-09-14)
It is well known that high-fat diets (HFDs) induce obesity and result in an increase in oxidative stress in adipose tissue, which leads to an impairment of fat mobilization by a downregulation of the lipases, such as hormone-sensitive lipase (HSL)
Nili Elinson et al.
The British journal of nutrition, 96(4), 691-696 (2006-10-03)
Leptin, a metabolic regulator of energy expenditure, exerts its peripheral effects primarily by altering lipid metabolism. The exocrine pancreas has a key role in the digestion of dietary lipids, but the role of leptin in regulating pancreatic lipases remains unknown.
J Hall et al.
The Journal of small animal practice, 54(5), 275-279 (2013-02-13)
Mushroom toxicosis is rarely diagnosed in dogs and is poorly reported in the veterinary literature. This report suggests that mushroom toxicosis is a potentially under-diagnosed condition in first opinion practice in the UK. Nine dogs with clinical signs consistent with
Minshan Hu et al.
Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 21(6), 751-757 (2013-03-05)
Diet induces changes in plasma lipid profiles, and the plasma lipid profiles vary among different genetic backgrounds. The aim of this study was to investigate how a high-carbohydrate (high-CHO) diet interacts with hepatic lipase G-250A promoter polymorphism to affect the

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