The antibiotics in the LB-agar plates are not stable. The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate. Because of this, we recommend the plates are used as soon as possible once they are received. For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).
L0418
LB Agar Carbenicillin - 100, Plates
LB Agar Carbenicillin - 100, Plates
pre-poured agar plates with 100 μg/ml carbenicillin
Synonym(s):
carbenicillin agar plates
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10 EA
$50.70
About This Item
UNSPSC Code:
41121800
sterility:
sterile; autoclaved
suitability:
selective for Escherichia coli
selective for coliforms
selective for coliforms
Recommended Products
grade
for molecular biology
Quality Level
sterility
sterile; autoclaved
form
plates (10 cm)
plates (10cm, 20-25ml each)
availability
available only in USA
capacity
20-25 mL
storage temp.
2-8°C
suitability
selective for Escherichia coli
selective for coliforms
Related Categories
General description
Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
Application
LB Agar Carbenicillin - 100, Plates may be used to culture transformed bacteria with an antibiotic-resistant plasmid using a microwave oven.[1]
Suitable for selective cultivation of E. coli strains that contain plasmids conferring carbenicillin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins.
Features and Benefits
Miller′s LB Agar plates provide:
- Convenient, ready-to-use 10cm format
- Standard formulation plus antibiotic
Components
15 g/L Agar
10 g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract
100 μg/ml carbenicillin
10 g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract
100 μg/ml carbenicillin
Other Notes
The plates must be used within two months of the manufacture date to ensure the antibiotics are fully active. The product may be shipped up until its expiration date but is generally shipped 2-4 weeks before expiration.
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Description
Pricing
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Sterile preparation of antibiotic-selective LB agar plates using a microwave oven
Iacoviello Michael P and Rubin SA
Biotechniques, 30(5), 963-965 (2001)
Articles
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
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Why do the LB-agar plates with antibiotics have such a short expiration date?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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