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F5022

Sigma-Aldrich

Fibronectin-like Protein Polymer genetically engineered

lyophilized powder, autoclaved, BioReagent, suitable for cell culture

Synonym(s):

ProNectin® F

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About This Item

MDL number:
MFCD00131067
UNSPSC Code:
12352200

sterility

autoclaved

Quality Level

200

product line

BioReagent

form

lyophilized powder

mol wt

75 kDa by gene sequence (differences are due to the unusual amino acid composition of the protein)
 ~110 kDa by SDS-PAGE

packaging

pkg of 1 mg
pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

solubility

4.5  M LiClO4: 1 mg/mL (stable for 12 mo. at RT.)

shipped in

dry ice

storage temp.

room temp

Application

Recommended as a cell culture substratum at 2-10 μg/cm2.

Packaging

Supplied with diluent in separate vial.

Other Notes

Positively-charged, protein polymer which incorporates multiple copies of the RGD cell attachment epitope from human fibronectin between repeated structural peptide units.

Reconstitution

Dilutions can be made to a working concentration with balanced salt solution; stable up to 2 days at RT. Coated labware is stable at least 4 mos at RT.

Legal Information

ProNectin is a registered trademark of Sanyo Chemical Industries, Ltd

pictograms

Flame over circleExclamation mark
GHS03,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Ox. Liq. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

5.1B - Oxidizing hazardous materials

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLBZ1125
SLBZ2856
SLBX2897
SLBX0834
SLBW8243

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Receptor-specific serum-free cell attachment using a highly stable engineered protein polymer.
Esty, A.
American Biotechnology Laboratory, 44-44 (1991)
Zoltán Jakus et al.
The Journal of experimental medicine, 206(3), 577-593 (2009-03-11)
beta(2) integrins and Fcgamma receptors are critically involved in neutrophil activation at the site of inflammation. Both receptor types trigger a receptor-proximal tyrosine phosphorylation cascade through Src family kinases and Syk, but further downstream signaling events are poorly understood. We
Polly A Machin et al.
Frontiers in immunology, 14, 1180886-1180886 (2023-06-29)
Rac-GTPases and their Rac-GEF activators play important roles in neutrophil-mediated host defence. These proteins control the adhesion molecules and cytoskeletal dynamics required for neutrophil recruitment to inflamed and infected organs, and the neutrophil effector responses that kill pathogens. Here, we
Lamyaa Shaban et al.
PLoS pathogens, 16(5), e1008576-e1008576 (2020-05-12)
Yersinia suppress neutrophil responses by using a type 3 secretion system (T3SS) to inject 6-7 Yersinia effector proteins (Yops) effectors into their cytoplasm. YopH is a tyrosine phosphatase that causes dephosphorylation of the adaptor protein SKAP2, among other targets in

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