CMC0023

CONTROLLER SIG10 Chemically Competent Cells

Escherichia coli, rod shaped

• UNSPSC Code: 12352200
• NACRES: NA.85

Properties

• product name: CONTROLLER SIG10 Chemically Competent Cells, for controlled protein expression
• biological source: Escherichia coli
• grade: for molecular biology
• growth mode: adherent or suspension
• morphology: rod shaped
• technique(s): microbiological culture: suitable
• cell transformation: competent cell type: chemically competent; transformation efficiency: >1 × 10⁹ cfu/μg
• shipped in: dry ice
• Storage temp.: −70°C

Description

General description

The CONTROLLER SIG10 Chemically Competent Cells are an E. coli strain optimized for high efficiency transformation.
They are ideal for cloning and propagation of plasmid clones. They give high yield and high quality plasmid DNA due to the endA1 mutation.

Genotype

mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ (ara,leu)7697 galU galK rpsL (StrR) nupG λ- tonA Mini-F lacIq1 (GentR)

Features and Benefits

• Reduce leaky expression: The LacI repressor protein binds to the lacO operator to block transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter will be greatly reduced.
• High protein yields: Both 10G and BL21(DE3)-based strains are noted for their ability to express high levels of protein. For expression from a T7 promoter please select the BL21(DE3) strain.

This strain also is deficient in proteases, yielding more stable protein preparations. Most other promoters can be used in either of the CONTROLLER strain.

• Terrific efficiency: The simple, reliable heat-shock procedure for chemical transformation gives excellent results from small amounts of plasmid DNA.

Components

• CONTROLLER SIG10 chemically competent cells
• pUC 19 transformation control DNA
• recovery medium for expression

Safety Information

• Storage Class: 10 - Combustible liquids