C3657

Human Collagen Type V

from human placenta, powder, suitable for detection assays

• CAS Number: 9007-34-5
• EC Number: 232-697-4
• MDL number: MFCD00130825
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• product name: Collagen from human placenta, Bornstein and Traub Type V (Sigma Type IX), powder
• biological source: human placenta
• Quality Level: 200
• form: powder
• impurities: HIV, hepatitis B and hepatitis C, none detected
• solubility: aqueous acid: soluble
• UniProt accession no.: P20908
• application(s): detection
• storage temp.: 2-8°C
• Gene Information: human ... COL5A1(1289)

Description

Components

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue.

Caution

This product should be stored desiccated at -20°C, and will retain activity in these conditions for 3 years.

Preparation Note

This product was prepared by a modification of the pepsin extraction and salt fractionation method of Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984). It is an acid soluble collagen that can be dissolved in water with acetic acid added to pH 3.0 (5 mg/mL), yielding an opalescent, colorless solution.

Analysis Note

An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)