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C2488

Sigma-Aldrich

Citrate Buffer Solution, 0.09 M

Synonym(s):

Citric Acid Buffer Solution

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100 ML
$58.42
500 ML
$198.55

$58.42

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100 ML
$58.42
500 ML
$198.55

About This Item

UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25

$58.42

List Price$68.60Save 15%

In StockDetails

Request a Bulk Order

description

Contains 1% chloroform
pH 4.8 at 25 °C.

Quality Level

200

form

solution

concentration

0.09 M

pH

4.8 (25 °C)

application(s)

diagnostic assay manufacturing

Storage temp.

2-8°C

Related Categories

General description

Citrate Buffer Solution, 0.09 M, is a high-quality biochemical buffer solution commonly employed in various research applications. Its applications include use in acid phosphatase reactions alongside p-nitrophenyl phosphate (pNPP) enzyme substrate. Citrate Buffer is also used for the isolation of RNA from cells and tissues.[1] In addition, it is also used as a background electrolyte for separating sulfonamides utilizing capillary zone electrophoresis.[2] With its superior buffering capacity and ability to maintain a stable pH, Citrate Buffer Solution, 0.09 M is an excellent choice for any researcher in need of reliable performance.

Application

Citrate Buffer Solution, 0.09 M has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections during immunohistochemistry (IHC) experiments.[3][4] It has also been used in the preparation of hybridization solutions for fluorescence in situ hybridization (FISH) procedures.[5]

Other Notes

Unit definition: One unit of acid phosphatase will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per, minute at pH 4.8 at 37 °C.

Pictograms

Health hazard
GHS08

signalword

Warning

hcodes

H351,H373

Hazard Classifications

Carc. 2 - STOT RE 2 Oral

target_organs

Liver,Kidney

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
0000334231
0000334229
0000334231
0000334229
SLCQ9536

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Migration behavior and separation of sulfonamides in capillary zone electrophoresis. I. Influence of buffer pH and electrolyte modifier
Lin CE, et al.
Journal of Chromatography A, 755(2), 261-269 (1996)
Argyrios G Daskalopoulos et al.
Oral surgery, oral medicine, oral pathology and oral radiology, 129(5), 493-513 (2020-03-17)
Toll-like receptors (TLRs) may promote or inhibit tumor progression. The aim of this study was to assess the expression of TLR4 and TLR9 and their downstream targets in oral tongue squamous cell carcinoma (OTSCC) in correlation with histopathologic parameters and
I Gkouveris et al.
Histology and histopathology, 32(10), 1065-1076 (2017-01-17)
Signal transducer and activator of transcription 3 (STAT3) and mitogen activated protein kinases (MAPKs), including ERK and JNK, have been implicated in oral squamous cell carcinoma (OSCC) development and progression. Our purpose was to evaluate the levels of activated STAT3
Nikolaos G Nikitakis et al.
Oral surgery, oral medicine, oral pathology and oral radiology, 125(2), 164-171 (2017-12-19)
Primary oral malignant melanoma (POMM) is a rare type of malignancy with a very poor prognosis, the molecular pathogenesis of which remains elusive. The aim of this study was to assess the expression status of signal transducer and activator of
Greg C Bristow et al.
Cell reports, 31(3), 107536-107536 (2020-04-23)
Chromosome 16p11.2 duplications dramatically increase risk for schizophrenia, but the mechanisms remain largely unknown. Here, we show that mice with an equivalent genetic mutation (16p11.2 duplication mice) exhibit impaired hippocampal-orbitofrontal and hippocampal-amygdala functional connectivity. Expression of schizophrenia-relevant GABAergic cell markers
View All Related Papers

Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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