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A1733

Sigma-Aldrich

AMPK (α2/β1/γ1), active, His tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

AMK2, AMPKG, HAMPKb, MGC17785, MGC8666, PRKAA2, PRKAB1, PRKAG1

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in baculovirus infected Sf9 cells

Quality Level

200

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

230-311 nmol/min·mg

mol wt

A2 subunit ~69 kDa
B1 subunit ~38 kDa
G1 subunit ~40 kDa

UniProt accession no.

P54619
P54646
Q9Y478

relevant disease(s)

diabetes

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKAA2(5563) , PRKAB1(5564) , PRKAG1(5571)

Biochem/physiol Actions

AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for the treatment of diabetes. AMPK is viewed as a fuel sensor for glucose and lipid metabolism by modulating the activity of the autonomous nervous system in vivo. Short-term overexpression of a constitutively active form of AMPK in the liver leads to mild hypoglycemia and fatty liver due to increased fatty acid utilization.

Physical form

Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

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Marc Foretz et al.
Diabetes, 54(5), 1331-1339 (2005-04-28)
AMP-activated protein kinase (AMPK) is a major therapeutic target for the treatment of diabetes. We investigated the effect of a short-term overexpression of AMPK specifically in the liver by adenovirus-mediated transfer of a gene encoding a constitutively active form of
B Viollet et al.
Biochemical Society transactions, 31(Pt 1), 216-219 (2003-01-28)
AMP-activated protein kinase (AMPK) is viewed as a fuel sensor for glucose and lipid metabolism. To understand better the physiological role of the catalytic AMPK subunit isoforms, we generated two knockout mouse models with the alpha1 (AMPK alpha 1(-/-)) and
Yanan Wang et al.
Nature communications, 13(1), 7215-7215 (2022-11-27)
Tumour cell metabolic plasticity is essential for tumour progression and therapeutic responses, yet the underlying mechanisms remain poorly understood. Here, we identify Prospero-related homeobox 1 (PROX1) as a crucial factor for tumour metabolic plasticity. Notably, PROX1 is reduced by glucose

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