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Key Documents

56822

Sigma-Aldrich

Immersion oil

for microscopy

Synonym(s):

immersion medium for light microscopy

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About This Item

MDL number:
MFCD00131369
UNSPSC Code:
12171500
NACRES:
NA.47

biological source

synthetic

Quality Level

100

grade

for microscopy

form

liquid

refractive index

n20/D 1.516

viscosity

100-120 mPa.s(20 °C)

density

1.025 g/mL at 20 °C

application(s)

hematology
histology

storage temp.

room temp

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General description

Immersion oil is a clear, viscous liquid with optimized refractive properties, specifically modified to closely approximate the refractive index (RI) of glass (ne = 1.5). It is used in conjunction with an objective lens to enhance the resolving power.

Application

Immersion oil is used for high-resolution (1000X) light microscopy work in conjunction with an oil immersion objective lens to optimize microscopic examinations of histological, cytological, hematological, and bacterial specimen material after it has been fixed, embedded, stained, or counterstained, and mounted.

Principle

Immersion oil is applied dropwise to stained and mounted or non-mounted specimen material to form a clear film between the specimen and the microscope lens to eliminate the deflection of incident light and thus substantially enhance the optical efficiency of the lens.

pictograms

Environment
GHS09

signalword

Warning

hcodes

H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

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Theis Sommer et al.
Scientific reports, 8(1), 13104-13104 (2018-09-01)
The catalytic mechanism of the cyclic amidohydrolase isatin hydrolase depends on a catalytically active manganese in the substrate-binding pocket. The Mn2+ ion is bound by a motif also present in other metal dependent hydrolases like the bacterial kynurenine formamidase. The
Thomas P Burghardt et al.
Applied optics, 48(32), 6120-6131 (2009-11-12)
Total internal reflection fluorescence (TIRF) microscopy uses the evanescent field on the aqueous side of a glass/aqueous interface to selectively illuminate fluorophores within approximately 100 nm of the interface. Applications of the method include epi-illumination TIRF, where the exciting light
P L Appleton et al.
Journal of microscopy, 234(2), 196-204 (2009-04-29)
Visualizing overall tissue architecture in three dimensions is fundamental for validating and integrating biochemical, cell biological and visual data from less complex systems such as cultured cells. Here, we describe a method to generate high-resolution three-dimensional image data of intact

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