Skip to Content
MilliporeSigma
All Photos(1)

Documents

45359

Sigma-Aldrich

Epoxy Embedding Medium kit

embedding resin for electron microscopy

Synonym(s):

Epon substitute embedding medium kit

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12171500
NACRES:
NA.47

pH

5-7

density

1.9 g/cm3

application(s)

hematology
histology

storage temp.

2-8°C

General description

The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

Application

Epoxy Embedding Medium Kit has been used in the following applications:

  • to study the role of NDRG2, an essential colonic epithelial barrier regulator, in gut homeostasis maintenance and colitis-associated tumor development
  • to study the interaction of polymer scaffold nanofibers with growing cells
  • to study the morphological characterization of postembryonic development of blood–spleen barrier in ducks
  • to understand the role of NOD1/NOD2 receptors in Fusobacterium nucleatum-mediated neutrophil extracellular traps

Features and Benefits

  • Excellent medium for embedding a wide variety of tissues, including both plant and animal.
  • Facilitates rapid embedding (less than 3 hours).
  • Low viscosity facilitates rapid tissue penetration.
  • Desirable hardness is easy to obtain to suit a specific tissue type.

Components

45345 Epoxy Embedding Medium 2x250 ml
45347 Hardener MNA 250 ml
45346 Hardener DDSA 250 ml
45348 Accelerator DMP 30 250 ml

Legal Information

Epon is a trademark of Hexion, Inc.

Related product

Product No.
Description
Pricing

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

275.0 °F

flash_point_c

135 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

A Sawaguchi et al.
Journal of microscopy, 234(2), 113-117 (2009-04-29)
The goal of specimen preparation for transmission electron microscopy is to obtain high-quality ultra-thin sections with which we can correlate cellular structure to physiological function. In this study, we newly developed a capsule-supporting ring that can be useful for resin
Konstantin E Mochalov et al.
Ultramicroscopy, 182, 118-123 (2017-07-04)
In the past decade correlative microscopy, which combines the potentials of different types of high-resolution microscopies with a variety of optical microspectroscopy techniques, has been attracting increasing attention in material science and biological research. One of outstanding solutions in this
Arnold Lavaud et al.
Translational vision science & technology, 9(5), 13-13 (2020-08-22)
To evaluate visual streak (VS) identification on spectral-domain optical coherence tomography (SD-OCT) scans in awake rabbits. To report thickness measurements in the VS and adjacent retina on OCT B-scans and histologic sections and to assess inter-method bias, precision and repeatability
Kunio Nagashima et al.
Methods in molecular biology (Clifton, N.J.), 697, 83-91 (2010-12-01)
This chapter outlines the procedures for ex vivo TEM preparation of nanoparticle-containing tissue or cell culture samples using an epoxy resin embedding method. The purpose of this procedure is to preserve the structure of tissue in a hardened epoxy block
Role of NOD1/NOD2 receptors in Fusobacterium nucleatum mediated NETosis
Alyami, et al.
Microbial Pathogenesis (2019)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service