11119915001
Roche
RNase, DNase-free
from bovine pancreas
Synonym(s):
Rnase
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About This Item
Recommended Products
biological source
bovine pancreas
Quality Level
form
solution
specific activity
≥30 units/mg protein
packaging
pkg of 500 μg (1 ml)
manufacturer/tradename
Roche
technique(s)
DNA purification: suitable
storage temp.
−20°C
Related Categories
General description
Application
Unit Definition
One unit produces a decrease in absorbance at 260 nm, which is equivalent to a total conversion of RNA to oligonucleotides in one minute at +25 °C.
Physical form
Preparation Note
- For small-scale isolation of plasmid DNA ("miniprep" from a 1.5 ml bacterial culture), use 0.5 μl of RNase, DNase-free in a reaction volume of 50 μl.
- To isolate plasmid DNA from a 100 ml bacterial culture, use 8 μl of RNase, DNase-free in a reaction volume of 2 ml.
- To isolate genomic DNA from cultured mammalian cells (5 x 107 cells), use 8 μl of RNase, DNase-free in a reaction volume of 2 ml.
Working solution: Storage and Dilution Buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0.
Other Notes
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
No data available
flash_point_c
No data available
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Protocols
0.1 mU RNase, DNase-free degrades 1 μg RNA in 30 min at + 37 °C in a reaction volume of 50 μL PCR grade water. The protein concentration of RNase, DNase-free is 0.5 μg/μL.
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