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11119915001

Roche

RNase, DNase-free

from bovine pancreas

Synonym(s):

Rnase

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About This Item

UNSPSC Code:
41105600

biological source

bovine pancreas

Quality Level

form

solution

specific activity

≥30 units/mg protein

packaging

pkg of 500 μg (1 ml)

manufacturer/tradename

Roche

technique(s)

DNA purification: suitable

storage temp.

−20°C

General description

Pyrimidine-specific endoribonuclease that acts on single-stranded RNA. RNase, DNase-free, is a heterogeneous mixture of ribonucleases that has been prepared free of deoxyribonuclease activity according to the current Quality Control procedures. RNase, DNase-free, is particularly well suited for use in DNA isolation procedures. Before use, most RNase preparations must be boiled to remove DNase activity. This preparation of RNase does not need to be boiled; it can be used directly from the vial.

Application

RNase, DNase-free, efficiently removes contaminating RNA from plasmid or genomic DNA preparations.[1][2]

Unit Definition

One Kunitz unit is the amount of enzyme that causes a decrease in absorbance of A0 to A1 within one minute under the assay conditions. A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
One unit produces a decrease in absorbance at 260 nm, which is equivalent to a total conversion of RNA to oligonucleotides in one minute at +25 °C.

Physical form

Solution, 500 μg/ml, in 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (pH 7.0).

Preparation Note

Working concentration: The optimal working concentration for RNase, DNase free, is 2 to 5 μg/ml. The reaction volume will vary for different applications. Some suggested guidelines are given below:
  1. For small-scale isolation of plasmid DNA ("miniprep" from a 1.5 ml bacterial culture), use 0.5 μl of RNase, DNase-free in a reaction volume of 50 μl.
  2. To isolate plasmid DNA from a 100 ml bacterial culture, use 8 μl of RNase, DNase-free in a reaction volume of 2 ml.
  3. To isolate genomic DNA from cultured mammalian cells (5 x 107 cells), use 8 μl of RNase, DNase-free in a reaction volume of 2 ml.

Working solution: Storage and Dilution Buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

No data available

flash_point_c

No data available


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    Minoo Rassoulzadegan et al.
    Cells, 10(6) (2021-07-03)
    Local three-stranded DNA/RNA hybrid regions of genomes (R-loops) have been detected either by binding of a monoclonal antibody (DRIP assay) or by enzymatic recognition by RNaseH. Such a structure has been postulated for mouse and human telomeres, clearly suggested by
    Stacy M Horner et al.
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    Enterocytozoon bieneusi is the most common microsporidian associated with human disease, particularly in the immunocompromised population. In the setting of HIV infection, it is associated with diarrhea and wasting syndrome. Like all microsporidia, E. bieneusi is an obligate, intracellular parasite
    Heather J Kolpa et al.
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    Here we provide a brief review of relevant background before presenting results of our investigation into the interplay between scaffold attachment factor A (SAF-A), chromatin-associated RNAs, and DNA condensation. SAF-A, also termed heterogenous nuclear protein U (hnRNP U), is a
    Maartje J Vogel et al.
    Nature protocols, 2(6), 1467-1478 (2007-06-05)
    Understanding gene regulatory networks in mammalian cells requires detailed knowledge of protein-DNA interactions. Commonly used methods for genome-wide mapping of these interactions are based on chromatin immunoprecipitation. However, these methods have some drawbacks, such as the use of crosslinking reagents

    Protocols

    0.1 mU RNase, DNase-free degrades 1 μg RNA in 30 min at + 37 °C in a reaction volume of 50 μL PCR grade water. The protein concentration of RNase, DNase-free is 0.5 μg/μL.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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