Kindly review the user guide for these products at the following link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/360/134/pr05035-rev1018.pdf
UFC9100
Amicon® Ultra Centrifugal Filter, 100 kDa MWCO
sample volume 15 mL, regenerated cellulose membrane, MWCO 100 kDa
Synonym(s):
100 kDa MWCO, 15 mL sample volume, Centrifugal concentrator
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8 PKG
$128.00
24 PKG
$377.00
96 PKG
$1,470.00
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8 PKG
$128.00
24 PKG
$377.00
96 PKG
$1,470.00
About This Item
UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24
Recommended Products
material
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
Quality Level
sterility
non-sterile
product line
Amicon®
manufacturer/tradename
Amicon® Ultra
parameter
15 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
12.1 cm (4.807 in.)
diam.
2.97 cm
filtration area
7.6 cm2
volume
15 mL
pore size
100 kDa MWCO
shipped in
ambient
General description
Amicon® Ultra 15 mL centrifugal devices with Ultracel® 100,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, IgG (156 kDa) at 1 mg/ml initial concentration is retained at >90% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
Application
- Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipettor sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Related Content
Concentrate protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters.
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Please could you provide the manual for these kind of filters: Amicon Ultra 15 ml centrifugal filters
1 answer-
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Hellow. Are these tubes valid for lentivirus concentration?
1 answer-
Yes, the Amicon Ultra 100 kDa products may be used to concentrate lentivirus samples. For the operation of the Amicon Ultra tubes for lentivirus concentration, please refer to the product instructions of the virus concentration and purification kit FTLV00003 and FTAV00003 below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/403/218/ds1987en-ms.pdfIt may also be helpful to review the publication below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/273/325/an1066en-ms.pdfHelpful?
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Hello, What is the shelf-life of these filters before wetting the membrane (i.e. how long can these sit unused before the membrane loses its properties)?
1 answer-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services
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