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UFC9100

Millipore

Amicon® Ultra Centrifugal Filter, 100 kDa MWCO

sample volume 15 mL, regenerated cellulose membrane, MWCO 100 kDa

Synonym(s):

100 kDa MWCO, 15 mL sample volume, Centrifugal concentrator

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8 PKG
$128.00
24 PKG
$377.00
96 PKG
$1,470.00

$128.00


Estimated to ship onFebruary 18, 2025


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8 PKG
$128.00
24 PKG
$377.00
96 PKG
$1,470.00

About This Item

UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24

$128.00


Estimated to ship onFebruary 18, 2025


Request a Bulk Order

material

polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing

Quality Level

sterility

non-sterile

product line

Amicon®

manufacturer/tradename

Amicon® Ultra

parameter

15 mL sample volume

technique(s)

protein extraction: suitable
protein purification: suitable

L

12.1 cm (4.807 in.)

diam.

2.97 cm

filtration area

7.6 cm2

volume

15 mL

pore size

100 kDa MWCO

shipped in

ambient

General description

Amicon® Ultra 15 mL centrifugal devices with Ultracel® 100,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, IgG (156 kDa) at 1 mg/ml initial concentration is retained at >90% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.

Application

  • Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
  • Concentration of dilute or pre-purified proteins from column eluents.
  • Desalting, buffer exchange and protein dialysis.

Features and Benefits

  • Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%.
  • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes).
  • Heat-sealed membrane minimizes downstream extractables.
  • 100% integrity tested for reliable performance.
  • Convenient sample monitoring with translucent housing and volume gradations.
  • Direct pipettor sample access eliminates processing step to recover concentrate.
  • High concentration factors of 80–100X.

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

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Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.

Related Content

Concentrate protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters.

Questions

11–13 of 13 Questions  
  1. Please could you provide the manual for these kind of filters: Amicon Ultra 15 ml centrifugal filters

    1 answer
    1. Kindly review the user guide for these products at the following link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/360/134/pr05035-rev1018.pdf

      Helpful?

  2. Hellow. Are these tubes valid for lentivirus concentration?

    1 answer
    1. Yes, the Amicon Ultra 100 kDa products may be used to concentrate lentivirus samples. For the operation of the Amicon Ultra tubes for lentivirus concentration, please refer to the product instructions of the virus concentration and purification kit FTLV00003 and FTAV00003 below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/403/218/ds1987en-ms.pdf

      It may also be helpful to review the publication below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/273/325/an1066en-ms.pdf

      Helpful?

  3. Hello, What is the shelf-life of these filters before wetting the membrane (i.e. how long can these sit unused before the membrane loses its properties)?

    1 answer
    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services

      Helpful?

11–13 of 13 Questions  

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