Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~52-57 kDa isoforms of MYH.
Recognizes the MYH protein and several of its approximately 10 isoforms in PC12, Jurkat, and HeLa cells.
This Anti-MYH (33-51) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Paraffin Sections for the detection of MYH (33-51).
Immunogen
Human
a synthetic peptide (QEGRQKHAKNNSQAKPSAC) corresponding to amino acids 33-51 of human MYH
Application
Immunoblotting (1:1500)
Immunofluorescence (1:500)
Paraffin Sections (see application references)
Warning
Toxicity: Standard Handling (A)
Physical form
Undiluted serum.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control PC12, Jurkat, or HeLa cells
Other Notes
Detects MYH and several of its approximately ten isoforms. In Jurkat cells the ~52 kDa and ~53 kDa variants were found in the nuclear fraction while the ~57 kDa protein is found in the mitochondria. Antibody should be titrated for optimal results in individual systems.
Wang, G., et al. 2000. Nucleic Acids Res.28, 2135.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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