ABD124

Anti-Wnt3a Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Protein Wnt-3a
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.75

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse
• technique(s): immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_033548
• UniProt accession no.: P27467
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... WNT3A(89780) ; mouse ... Wnt3A(22416)

Description

General description

Wnt-3a, also known as Protein Wnt3a, and encoded by the gene name Wnt3a and Wnt-3a, is a ligand for members of the frizzled family of seven transmembrane receptors and a member of the Wnt family. The Wnt family of consists of ligands involved in cell development, fate determination, organogenesis, and oncogenesis. Information on Wnt signaling has been inferred from genetic analysis of wingless (wg) signaling in Drosophila. The Wnt signaling cascade initiates at the cell membrane when Wnt interacts with its receptor, a member of the Frizzled (Fz) transmembrane receptor family and lipoprotein receptor–related proteins 5 and 6 (LRP-5/6). This interaction relays the signal into the interior of the cell where disheveled (dsh) is brought to the membrane and gets phosphorylated/activated. Dsh promotes the inactivation of GSK3 kinase activity, thus preventing the phosphorylation of β-Catenin and allowing it to accumulate as it avoids ubiquination and subsequent proteosome degradation. Stable β-Catenin accumulates in the cytoplasm where it interacts with lymphoid enhancer factor/T cell factor (LEF/TCF) and translocates into the nucleus. This results in activation of TCF-responsive genes which are thought to play key roles in development and cancer. Negative regulators of the pathway may include axin and the tumor suppressor APC. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Wnt-3a is expressed in dorsal portion of the neural tube (developing roof plate), and mesenchyme tissue surrounding the umbilical veins.

Immunogen

Linear peptide corresponding to mouse Wnt3a.

Application

Immunocytochemistry Analysis: A representative lot detected Wnt3a in COS7 cell lysate expressing WT Wnt3a (Kishida, S., et al. (2004). Mol Cell Biol. 10:4487-501).
Western Blotting Analysis: A representative lot detected Wnt3a in Cos-7 cell lysate overexpressing Wnt-3a (Sato, A., et al. (2010). EMBO J. 29(1):41-54).

Research Category
Stem Cell Research

This Anti-Wnt3a antibody is validated for use in WB, IC for the detection of Wnt3a.

Quality

Evaluated by Western Blotting in Cos 7 overexpressing mouse cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Wnt3a in 10 µg of Cos 7 overexpressing mouse cell lysate.

Target description

~39 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable