05-257

Anti-p300 CT Antibody, clone RW128

clone RW128, Upstate^®, from mouse

• Synonym(s): KAT3B
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: RW128, monoclonal
• species reactivity: mouse, rat, human
• manufacturer/tradename: Upstate^®
• technique(s): ChIP: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_001429
• UniProt accession no.: Q09472
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... EP300(2033)

Description

General description

p300 (also EP300 or E1A binding protein p300) and CBP (also CREBBP or CREB binding protein) are two closely related transcriptional co-activating proteins (or coactivators). p300 and CBP interact with numerous transcription factors and act to increase the expression of their target genes.
p300 and CBP have similar structures. Both contain five protein interaction domains, the nuclear receptor interaction domain (RID), the CREB and MYB interaction domain (KIX), the cysteine/histidine regions (CH1 and CH3) and the interferon response binding domain (IBiD). In addition p300 and CBP each contain a protein or histone acetyltransferase (PAT/HAT) domain and a bromodomain that binds acetylated lysines and a PHD finger motif with unknown function.
p300 and CBP are thought to increase gene expression in three ways:
(1) by relaxing the chromatin structure at the gene promoter through their intrinsic histone acetyltransferase (HAT) activity
(2) by recruiting the basal transcriptional machinery including RNA polymerase II to the promoter
(3) by acting as adaptor molecules.

Specificity

Recognizes p300 and co-immunoprecipitates E1A.

Immunogen

Epitope: C-terminus

GST-fusion to the C-Terminus of human p300

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Chromatin Biology

RNA Binding Protein (RBP)

Use Anti-p300 CT Antibody, clone RW128 (Mouse Monoclonal Antibody) validated in ChIP, IP, WB to detect p300 CT also known as KAT3B.

Quality

routinely evaluated by immunoblot on HeLa nuclear extract

Target description

300 kDa

Physical form

Format: Purified

Protein A Purified immunoglobulin in Protein A Purified immunoglobulin in PBS, pH 7.4, 0.05% sodium azide and 30% glycerol

Protein G Purified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable