The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Components
The supplements contain all of the following components except as indicated: Amino acids: All standard amino acids except for histidine. All the amino acids are present at a concentration of 76 mg/lL except for leucine, which is present at 380 mg/L. Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), uracil (76 mg/L), p-aminobenzoic acid (8 mg/L).
Other Notes
Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.
Cellular proteostasis is maintained via the coordinated synthesis, maintenance, and breakdown of proteins in the cytosol and organelles. While biogenesis of the mitochondrial membrane complexes that execute oxidative phosphorylation depends on cytoplasmic translation, it is unknown how translation within mitochondria impacts
Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.
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