SRP6176
Jacalin
recombinant, expressed in Artocarpus heterophyllus (jackfruit), ≥97% (SDS-PAGE)
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
biological source
Artocarpus integrifolia seeds
recombinant
expressed in Artocarpus heterophyllus (jackfruit)
assay
≥97% (SDS-PAGE)
form
lyophilized
mol wt
14.6 kDa (β chain)
17.1 kDa (full protein)
packaging
pkg of 5 mg
technique(s)
immunohistochemistry: suitable
impurities
<0.1EU/mg
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
General description
Jacalin is isolated from Jackfruit seeds and purified by affinity chromatography. It is a glycoprotein with two non-covalently bound subunits (14.6 and 17.1 kDa) and belongs to the family of galactose-binding lectins. Jacalin is preferably used in applications to isolate IgA from human serum, isolating human plasma glycoproteins and for applications in histochemistry. BioVision′s Jacalin is supplied as a white to light-yellow lyophilized powder without additives. The purity of the lectin is determined by SDS-PAGE, generating two homogeneous bands at 14 kDa and 17 kDa. Gene Bank Accession Number Q38720.
Biochem/physiol Actions
Jacalin is one of the two lectins found in jackfruit seeds. The other lectin is artocarpin. Jacalin has been used to inhibit herpes simplex virus type 2. It has been used to assess the immune status of patients infected with human immunodeficiency virus 1 (HIV1) as it exhibits mitogenic effect on human CD4+T lymphocytes. It has been used to analyze 0-linked glycoproteins due to its high specificity for O-glycoside of the disaccharide Thomsen-Friedenreich antigen (Galβ1-3GalNAc).[1]
Physical form
Lyophilized without additives
Preparation Note
Centrifuge the vial prior to opening.
Reconstitution
Reconstitute in water.
Other Notes
MAYSSLLSLS VLALLFSISS ADTRKWFLAN GINQNPIGII EAAVGVSEDL LNLNGMEAKN KSQTV IVGPWGAQVS TSSNGKAFDD GAFTGIREIN LSYNKETAIG DFQVIYDLNG SPYVGQNHTS FIKGFTPVKI SLDFPSEYIV DVSGYTGKVS GYVVVRSLTF KTNKKTYGPY GVTSGTPFNL PIENGLVVGF KGSIGYWLDY FSMYLSL
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Jackfruit and its many functional components as related to human health: a review.
Swami SB
Comprehensive Reviews in Food Science and Food Safety, 11, 565-576 (2012)
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