SAB4502019

Anti-NOTCH1 (Cleaved-Val¹⁷⁵⁴) antibody produced in rabbit

affinity isolated antibody

• Synonym(s): NOTC1, Notch homolog 1, neurogenic locus notch 1, notch 1 (translocation-associated), translocation-associated (Drosophila) (TAN1)
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 110 kDa
• species reactivity: rat, mouse, human
• concentration: ~1 mg/mL
• technique(s): ELISA: 1:5000; immunohistochemistry: 1:50-1:100; western blot: 1:500-1:1000
• NCBI accession no.: P46531
• UniProt accession no.: P46531
• application(s): research pathology
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: proteolytically cleaved (Val1754)
• Gene Information: human ... NOTCH1(4851)

Description

General description

The NOTCH1 gene is mapped to human chromosome 9q34 and encodes a member of the NOTCH family of proteins. The gene encodes a large transmembrane protein that contains an extracellular domain with 36 tandem epidermal growth factor (EGF)-like repeats and three cysteine-rich Notch/LIN-12 repeats, a six ankyrin repeat-containing intracellular domain, and a transactivation domain.

Anti-NOTCH1 (Cleaved-Val¹⁷⁵⁴) Antibody detects endogenous levels of fragment of activated NOTCH1 (Cleaved-Val¹⁷⁵⁴) protein.

Immunogen

The antiserum was produced against synthesized peptide derived from human Notch 1.

Immunogen Range: 1735-1784

Application

Anti-NOTCH1 (Cleaved-Val1754) antibody produced in rabbit has been used in immunohistochemistry (IHC).

Biochem/physiol Actions

The Notch1 protein is a transmembrane receptor that induces pluripotent progenitor cells to form T-cells and facilitates the assembly of pre–T cell receptor complexes in immature thymocytes. It participates in an intracellular signaling pathway that regulates cellular differentiation, cell fate and lateral inhibition. Mutations in this gene have been associated with human blood cancers, abnormal cardiovascular calcification, aortic valve disease, and human T cell acute lymphoblastic leukemia.

Features and Benefits

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Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable