It is crucial to adhere to the instructions for the Reticulocyte Stain Protocol (R4132) precisely. 10 minutes means 10 minutes. If you exceed 10 minutes, the test results may be inaccurate. The expectation is that 10 minutes was chosen to minimize the number of false positive results. Incubating the slides longer than 10 minutes is not recommended.
Recommended Products
form
solution
Quality Level
shelf life
Expiry date on the label.
IVD
for in vitro diagnostic use
technique(s)
microbe id | staining: suitable
application(s)
hematology
histology
storage temp.
room temp
Application
Utilizes New Methylene Blue for the identification of reticulocytes in blood films. Reticulocyte Stain has been used in the detection of reticulocytes.[1]
Principle
Blood and reticulocyte staining solution (New Methylene Blue) are mixed and incubated briefly at room temperature. Wedge or spun smears are made on microscope slides, air dried and evaluated under oil immersion on a light microscope. A red blood cell scoring positive would be observed containing two or more blue-stained granules.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Can incubation time be longer than the 10 minutes specified in the Instructions For Use? If so, for how long? Also, in general, how much time can lapse between step 1 and step 2?
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