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R0883

Sigma-Aldrich

RPMI-1640 Medium

RPMI-1640 Medium

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Roswell Park Memorial Institute 1640 medium

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100 ML
$16.70
500 ML
$38.10
1 L
$60.90
6 X 500 ML
$196.00
6 X 1 L
$333.00
24 X 500 ML
$691.00

$16.70

Estimated to ship onMarch 24, 2025

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All Photos(1)

Select a Size

Change View
100 ML
$16.70
500 ML
$38.10
1 L
$60.90
6 X 500 ML
$196.00
6 X 1 L
$333.00
24 X 500 ML
$691.00

About This Item

MDL number:
MFCD00217820
UNSPSC Code:
12352207
NACRES:
NA.75

$16.70

Estimated to ship onMarch 24, 2025

Request a Bulk Order

Product Name

RPMI-1640 Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium has been used:
  • to culture PC-3 prostate cell line and Jurkat cells[1]
  • to maintain a triple-negative MDA-MB-231 and a luminal type MCF7 human Caucasian breast adenocarcinoma cell line[2]
  • to cultivate circulating tumor cells (CTCs)[3]

Reconstitution

Supplement with 0.3 g/L L-glutamine.

also commonly purchased with this product

Product No.
Description
Pricing

supplement

Product No.
Description
Pricing

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
RNBM1122
RNBM0262
RNBM0101
RNBM0100
RNBL9396

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M Mathur et al.
JNMA; journal of the Nepal Medical Association, 53(198), 108-112 (2016-03-20)
Dermatophytosis is a common superficial fungal infection of the skin, hair and nails caused by Trichophyton, Microsporum and Epidermophyton dermatophytic species. Identification of causative dermatophytic species tend to vary with time and place and antifungal sensitivity is of epidemiological concern
MUC1 antigen-specific CD8 T lymphocytes targeting MCF7 and MDA-MB-231 human breast adenocarcinoma cell lines
Parsonidis P, et al.
Journal of Cancer Therapy, 10(07), 495-495 (2019)
Stepan Coufal et al.
Cells, 8(7) (2019-07-25)
Crohn's disease (CD), ulcerative colitis (UC) and inflammatory bowel disease (IBD) associated with primary sclerosing cholangitis (PSC-IBD), share three major pathogenetic mechanisms of inflammatory bowel disease (IBD)-gut dysbiosis, gut barrier failure and immune system dysregulation. While clinical differences among them
Adela Ngwewondo et al.
PLoS neglected tropical diseases, 12(6), e0006565-e0006565 (2018-06-14)
Ivermectin is the only drug currently recommended for the treatment of onchocerciasis, the second leading infectious cause of blindness in the world. This drug kills only the first stage larvae-microfilariae (mf) of Onchocerca volvulus and is to be used cautiously
Nicholas R Meyerson et al.
PLoS pathogens, 14(3), e1006906-e1006906 (2018-03-09)
HIV-1 arose as the result of spillover of simian immunodeficiency viruses (SIVs) from great apes in Africa, namely from chimpanzees and gorillas. Chimpanzees and gorillas were, themselves, infected with SIV after virus spillover from African monkeys. During spillover events, SIV
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Protocols

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WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.

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MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

Cell Viability and Proliferation XTT Assay Protocol Guide

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

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Questions

  1. Could you tell me the exact concentration of glucose in this RPMI-1640 medium(cat#R0883 ), please ?

    1 answer

    1. For this RPMI formulation, the glucose concentration is 2 grams per liter.

      Please see the datasheet below which includes the formulations of all classical RPMI-1640 media products:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/229/770/r0883for.pdf

      Helpful?

Reviews

Active Filters

  1. Boston, MA
    • Reviews 3
    • Votes 9
    5 out of 5 stars.

    Great product!

    Reliable cell culture product that gets frequent use

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